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Invitrogen
CYP27B1 Polyclonal Antibody
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Application
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CYP27B1 Antibody (PA5-121400) in WB
Western Blot analysis of CYP27B1 using CYP27B1 Polyclonal Antibody (Product # PA5-121400). Positive WB detected in: 293 whole cell lysate. All lanes: CYP27B1 antibody at 1:2,000. Secondary. Goat polyclonal to rabbit IgG at 1/50,000 dilution. Predicted band size: 57 kDa. Observed band size: 57 kDa.
Product Details
PA5-121400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial protein (350-491aa)
Conjugate
Unconjugated
Form
Liquid
Concentration
2.01 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914972
Product Specific Information
Antibody tested in Indirect ELISA
Target Information
CYP27B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1alpha(OH)ase; 25 hydroxyvitamin D3-1-alpha hydroxylase; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase; 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial; 25-OHD-1 alpha-hydroxylase; Calcidiol 1-monooxygenase; cy; Cytochrome p450 27B1; Cytochrome P450 subfamily XXVIIB polypeptide 1; cytochrome P450, family 27, subfamily B, polypeptide 1; Cytochrome P450C1 alpha; Cytochrome P450VD1-alpha; VD3 1A hydroxylase
Gene Aliases: CP2B; CYP1; CYP1ALPHA; CYP27B; CYP27B1; P450c1; PDDR; VDD1; VDDR; VDDRI; VDR
UniProt ID: (Human) O15528
Entrez Gene ID: (Human) 1594
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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