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Invitrogen
Calpain 1 Polyclonal Antibody
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Application
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Calpain 1 Antibody (PA5-78911) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of Calpain 1 in A549 cells using Calpain 1 Polyclonal Antibody (Product # PA5-78911). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 di... View More
Product Details
PA5-78911
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Calpain 1 (312-326aa EWNNVDPYERDQLRV).
Conjugate
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746027
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human Jurkat whole cell, human RT4 whole cell, human A431 whole cell, rat lung tissue, mouse lung tissue. IHC: rat lung tissue, human intestinal cancer tissue IHC-F: rat cardiac muscle tissue. ICC/IF: A549 cell, HELA Cell.
Target Information
The calpains including Calpain 1 (CAPN1), calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. Diseases associated with CAPN1 include Spastic Paraplegia 76, Autosomal Recessive and Spasticity.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Calcium-activated neutral proteinase 1; calpain 1, (mu/I) large subunit; Calpain mu-type; calpain, large polypeptide L1; Calpain-1 catalytic subunit; Calpain-1 large subunit; Calpain-I; Calpain-II; CANP 1; Cell proliferation-inducing gene 30 protein; cell proliferation-inducing protein 30; Micromolar-calpain; mu-calpain; muCANP
Gene Aliases: CANP; CANP1; CANPL1; Capa-1; Capa1; CAPN1; Cls1; mu-calpin; muCANP; muCL; PIG30; SPG76
UniProt ID: (Human) P07384, (Mouse) O35350, (Rat) P97571
Entrez Gene ID: (Human) 823, (Mouse) 12333, (Rat) 29153
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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