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Calpain 6 Antibody (PA5-89430) in WB
Western blot analysis of Calpain 6 in extracts of rat heart. Samples were incubated with Calpain 6 Polyclonal antibody (Product # PA5-89430) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-89430
产品规格
种属反应
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CAPN6 (NP_0551042)
偶联物
Unconjugated
形式
Liquid
浓度
1.14 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2805567
产品详细信息
Immunogen sequence: MGPPLKLFKN QKYQELKQEC IKDSRLFCDP TFLPENDSLF YNRLLPGKVV WKRPQDICDD PHLIVGNISN HQLTQGRLGH KPMVSAFSCL AVQESHWTKT IPNHKEQEWD PQKTEKYAGI FHFRFWHFGE WTEVVIDDLL PTINGDLVFS FSTSMNEFWN ALLEKAYAKL LGCYEALDGL TITDIIVDFT GTLAETVDMQ KGRYTELVEE KYKLFGELYK TFTKGGLICC SIESPNQEEQ EVETDWGLLK GHTYTMTDIR KIRLGERLVE; Positive Samples: Rat heart; Cellular Location: Cytoplasm, cytoskeleton, perinuclear region, spindle
靶标信息
Calpain-6, also known as calpamodulin or calpain-X, is an intracellular, calcium-dependent cysteine protease. Calpain-6 has a much more tissue-specific expression in adults than the ubiquitous calpain-1 and calpain-2, and has thus far been found primarily in the placenta, although it is expressed embryonically in a number of tissues. The classical calpain family members consist of a common small subunit (calpain-4), and a large variable subunit, but it is not known if calpain-6 associates with a small subunit. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV (domain T in calpains 5 and 6). Located on the X chromosome, the calpain-6 sequence lacks the "EF hand" calcium-binding motif found in domain-IV of the classical calpains. In addition, the canonical active site Cys, His and Asn are modified to Lys, His, Asn in human and Lys, Tyr, Asn in mouse calpain-6, making it unlikely that calpain-6 is proteolytically active.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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