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Invitrogen
Carbonic Anhydrase VA Polyclonal Antibody
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Application
FIGURE: 1 / 1
Carbonic Anhydrase VA Antibody (PA5-89996) in WB
Western blot analysis of Carbonic Anhydrase VA in mouse liver. Samples were incubated with Carbonic Anhydrase VA Polyclonal antibody (Product # PA5-89996) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-89996
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 39-305 of human CA5A (NP_0017301)
Conjugate
Unconjugated
Form
Liquid
Concentration
2.05 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805883
Product Specific Information
Immunogen sequence: CAWQTSNNTL HPLWTVPVSV PGGTRQSPIN IQWRDSVYDP QLKPLRVSYE AASCLYIWNT GYLFQVEFDD ATEASGISGG PLENHYRLKQ FHFHWGAVNE GGSEHTVDGH AYPAELHLVH WNSVKYQNYK EAVVGENGLA VIGVFLKLGA HHQTLQRLVD ILPEIKHKDA RAAMRPFDPS TLLPTCWDYW TYAGSLTTPP LTESVTWIIQ KEPVEVAPSQ LSAFRTLLFS ALGEEEKMMV NNYRPLQPLM NRKVWASFQA TNEGTRS; Positive Samples: K-562, LO2, HepG2; Cellular Location: Mitochondrion
Target Information
Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA5A gene encodes for the CA V protein which is expressed in the mitochondrial matrix in liver tissues, but can also be detected in many other tissues. In ureagenesis, the CA V protein may be involved in providing bicarbonate to carbamoyl phosphate synthetase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CA Y; CA-VA; Carbonate dehydratase VA; carbonic anhydrase 5, mitochondrial; Carbonic anhydrase 5A, mitochondrial; carbonic anhydrase 5b, mitochondrial; Carbonic anhydrase VA
Gene Aliases: Ca5; Ca5a; Car5; Car5a; CAV
UniProt ID: (Mouse) P23589
Entrez Gene ID: (Mouse) 12352
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