Proteintech

Cardiac Troponin T Polyclonal Antibody

Cardiac Troponin T Antibody in Immunocytochemistry (ICC/IF)

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Cardiac Troponin T Antibody (15513-1-AP) in ICC/IF

Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (magenta, Cat. No Product # 15513-1-AP), CD31/PECAM-1 (white), and Arl13B (yellow, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (cyan). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to magenta.CD31 stains endocardial/endothe... View More

Cardiac Troponin T Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cardiac Troponin T Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Cardiac Troponin T Antibody in Western Blot (WB)

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Product Details

15513-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 24 publications 24 publications

Flow Cytometry (Flow)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Cardiac Troponin T Fusion Protein Ag7716 (1-285 aa encoded by BC002653)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSDIEEVVE EYEEEEQEEA AVEEQEEAAE EDAEAEAETE ETRAEEDEEE EEAKEAEDGP MEESKPKPRS FMPNLVPPKI PDGERVDFDD IHRKRMEKDL NELQALIEAH FENRKKEEEE LVSLKDRIER RRAERAEQQR IRNEREKERQ NRLAEERARR EEEENRRKAE DEARKKKALS NMMHFGGYIQ KTERKSGKRQ TEREKKKKIL AERRKVLAID HLNEDQLREK AKELWQSIYN LEAEKFDLQE KFKQQKYEIN VLRNRINDNQ KVSKTRGKAK VTGRWK (1-285 aa encoded by B C002653)

Target Information

Cardiac Troponin T is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Cardiac Troponin T is important in proper muscle function and myofibril formation. In the human heart, four cTnT isoforms are expressed in a developmentally regulated manner. Cardiac Troponin T expression in the human heart is affected by heart failure. Mutations within the Cardiac Troponin T protein have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for the Cardiac Troponin T gene undergo alternative splicing that results in many tissue-specific isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Bioinformatics

Protein Aliases: Cardiac muscle troponin T; cardiac TnT; cardiac troponin T2; cardiomyopathy, dilated 1D (autosomal dominant); cardiomyopathy, hypertrophic 2; cTnT; MGC3889; TnTc; Troponin T cardiac; troponin T type 2 (cardiac); Troponin T, cardiac muscle; Troponin T2; troponin T2, cardiac; truncated cardiac troponin T

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Gene Aliases: CMD1D; CMH2; CMPD2; cTnT; Ctt; CTTG; LVNC6; RATCTTG; RCM3; TNNT2; Tnnt3; Tnt; TnTC

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UniProt ID: (Human) P45379, (Rat) P50753, (Mouse) P50752

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Entrez Gene ID: (Human) 7139, (Rat) 24837, (Mouse) 21956

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Function(s)

actin binding protein binding tropomyosin binding troponin C binding protein binding, bridging troponin I binding structural constituent of cytoskeleton ATPase activity actin binding motor protein

Process(es)

regulation of heart contraction muscle filament sliding negative regulation of ATPase activity positive regulation of ATPase activity regulation of muscle filament sliding speed protein heterooligomerization response to calcium ion actin crosslink formation ventricular cardiac muscle tissue morphogenesis cardiac muscle contraction muscle contraction regulation of muscle contraction heart development metabolic process sarcomere organization atrial cardiac muscle tissue morphogenesis

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cardiac Troponin T Polyclonal Antibody

Cardiac Troponin T Antibody (15513-1-AP) in ICC/IF

Product Details

15513-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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