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Invitrogen
Cas9 Monoclonal Antibody (7A9)
Application
FIGURE: 1 / 7
Cas9 Antibody (MA5-23519) in ICC/IF
Immunofluorescent detection of Cas9 in Hela cells transiently transfected with a Flag-tagged Cas9 expression vector using a Cas9 monoclonal antibody (Product # MA5-23519). 48 hours post transfection, the cells were fixed in 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the Cas9 (fig 4A and B) or with an anti-Flag (fig 4C) antibody at 4°C overnight, followed by incubation with an anti-mouse secondary antibody coupled to AlexaFluor 488 for 1 hr at RT.... View More
Product Details
MA5-23519
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Published species
Bacteria,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
7A9
Immunogen
Cas9 nuclease (CRISPR-associated protein 9).
Conjugate
Unconjugated
Form
Liquid
Concentration
2 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.05% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610639
Product Specific Information
MA5-23519 is derived from Streptococcus pyogenes.
Target Information
Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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