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FIGURE: 1 / 2
Catalase Antibody (100-4151) in WB
Western Blot of Rabbit Anti-Catalase (Bovine Liver) Antibody. Lane 1: CHO K1 Lysate 10µg. Lane 2: CHO S Lysate 10µg. Lane 3: Opal Pre-stained Molecular Weight Marker (p/n MB-210-0500). Primary Antibody: Rabbit Anti-Catalase at 1:500 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG CY5 (p/n 611-110-122) at 1:10,000 for 30 in at RT. Expect: ~59.8kDa.
Product Details
100-4151
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Catalase Antibody was produced by repeated immunizations with bovine liver catalase protein.
Conjugate
Form
Lyophilized
Concentration
85 mg/mL
Storage buffer
0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Catalase [Bovine Liver]. Cross reactivity against Catalase from other tissues and species may occur but has not been specifically determined.
Target Information
Catalase is a homotetrameric heme-containing enzyme present within the matrix of all peroxisomes. It carries out a dismutation reaction in which hydrogen peroxide is converted to water and oxygen. Human catalase has the last four amino acids (-KANL) at the extreme C-terminus for peroxisome targeting. The monomer of human catalase is 61.3 kDa in molecular size. Catalase has been implicated as an important factor in inflammation, mutagenesis, prevention of apoptosis, and stimulation of a wide spectrum of tumors. Loss of catalase leads to the human genetic disease, acatalasemia, or Takahara's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Catalase; CTA1; HGNC:1516; MGC138422; MGC138424
Gene Aliases: CAT
UniProt ID: (Bovine) P00432
Entrez Gene ID: (Bovine) 531682
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