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Invitrogen

Cathepsin L Monoclonal Antibody (33-2), eBioscience™

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3 Published Figures

6 References

View all (45) Cathepsin L antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Application

Cathepsin L Antibody in Western Blot (WB)

FIGURE: 1 / 10

Cathepsin L Antibody (BMS1032) in WB

Knockout of Cathepsin L was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR1042354_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Cathepsin L was performed by loading 30 µg of A549 Cas9 (Lane 1), andA549 Cathepsin L KO (Lane 2) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlo... View More

Cathepsin L Antibody in Immunocytochemistry (ICC/IF)

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Product Details

BMS1032

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

5 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

Assay-Dependent

Product Specifications

Species Reactivity

Human

Published species

Human, Non-human primate

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

33-2

Immunogen

Rec. Human Cathepsin

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2-7.4

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10596643

Product Specific Information

Description: Cathepsin L is a lysosomal cysteine proteinase consisting of a heavy chain of about 25 kDa and a light chain of about 5 kDa derived proteolytically from the same precursor. Cathepsin L has been shown to be produced by many different cell types. Since cathepsin L is capable of degrading protein constituents of the extracellular matrix it is assumed to play a crucial role in tumor progression and metastasis and a number of other disorders where the destruction of the extracellular matrix is the major cause of disease (e.g. rheumatoid arthritis, neurodegeneration).

Applications Tested: ELISA, Immunohistochemistry (Frozen), Western Blotting.

Target Information

The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SARS-CoV-2 Enters Human Leydig Cells and Affects Testosterone Production In Vitro.

Cells

Li L,Sottas CM,Chen HY,Li Y,Cui H,Villano JS,Mankowski JL,Cannon PM,Papadopoulos V

BMS1032 was used in Western Blotting to show that SARS-CoV-2 can enter hLLCs through a distinct pathway and alter testosterone production.

Thu Apr 20 00:00:00 UTC 2023

Genome-scale CRISPR screens identify host factors that promote human coronavirus infection.

Genome medicine

Grodzki M,Bluhm AP,Schaefer M,Tagmount A,Russo M,Sobh A,Rafiee R,Vulpe CD,Karst SM,Norris MH

BMS1032 was used in Western Blot to substantiate and expand the growing body of literature focused on understanding key human coronavirus-host cell interactions and exploit that knowledge for rational antiviral drug development.

Thu Jan 27 00:00:00 UTC 2022

Genome-scale CRISPR screens identify host factors that promote human coronavirus infection.

Genome medicine

Grodzki M,Bluhm AP,Schaefer M,Tagmount A,Russo M,Sobh A,Rafiee R,Vulpe CD,Karst SM,Norris MH

Thu Jan 27 00:00:00 UTC 2022

SerpinB3 drives cancer stem cell survival in glioblastoma.

Cell reports

Lauko A,Volovetz J,Turaga SM,Bayik D,Silver DJ,Mitchell K,Mulkearns-Hubert EE,Watson DC,Desai K,Midha M,Hao J,McCortney K,Steffens A,Naik U,Ahluwalia MS,Bao S,Horbinski C,Yu JS,Lathia JD

Published figure using Cathepsin L monoclonal antibody (Product # BMS1032) in Immunocytochemistry

Tue Sep 13 00:00:00 UTC 2022

SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-β signaling.

Nature communications

Biering SB,Gomes de Sousa FT,Tjang LV,Pahmeier F,Zhu C,Ruan R,Blanc SF,Patel TS,Worthington CM,Glasner DR,Castillo-Rojas B,Servellita V,Lo NTN,Wong MP,Warnes CM,Sandoval DR,Clausen TM,Santos YA,Fox DM,Ortega V,Näär AM,Baric RS,Stanley SA,Aguilar HC,Esko JD,Chiu CY,Pak JE,Beatty PR,Harris E

BMS1032 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to offer mechanistic insight into SARS-CoV-2-triggered vascular leak, providing a starting point for development of therapies targeting COVID-19.

Fri Dec 09 00:00:00 UTC 2022

TMPRSS2 expression dictates the entry route used by SARS-CoV-2 to infect host cells.

The EMBO journal

Koch J,Uckeley ZM,Doldan P,Stanifer M,Boulant S,Lozach PY

BMS1032 was used in Western Blot to indicate that SARS-CoV-2 infects cells through distinct, mutually exclusive entry routes and highlight the importance of TMPRSS2 for SARS-CoV-2 sorting into either pathway.

Mon Aug 16 00:00:00 UTC 2021

TMPRSS2 expression dictates the entry route used by SARS-CoV-2 to infect host cells.

The EMBO journal

Koch J,Uckeley ZM,Doldan P,Stanifer M,Boulant S,Lozach PY

Mon Aug 16 00:00:00 UTC 2021

Obatoclax impairs lysosomal function to block autophagy in cisplatin-sensitive and -resistant esophageal cancer cells.

Oncotarget

Yu L,Wu WK,Gu C,Zhong D,Zhao X,Kong Y,Lin Q,Chan MT,Zhou Z,Liu S

Published figure using Cathepsin L monoclonal antibody (Product # BMS1032) in Western Blot

Tue Mar 22 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: Cathepsin L1; Major excreted protein; MEP; Procathepsin L

Gene Aliases: CATL; CTSL; CTSL1; MEP

UniProt ID: (Human) P07711

Entrez Gene ID: (Human) 1514

Function(s)

fibronectin binding cysteine-type endopeptidase activity serine-type endopeptidase activity protein binding collagen binding cysteine-type peptidase activity histone binding proteoglycan binding serpin family protein binding cysteine protease

Process(es)

toll-like receptor signaling pathway adaptive immune response proteolysis antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class II extracellular matrix disassembly collagen catabolic process proteolysis involved in cellular protein catabolic process macrophage apoptotic process cellular response to thyroid hormone stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cathepsin L Monoclonal Antibody (33-2), eBioscience™

Cathepsin L Antibody (BMS1032) in WB

Product Details

BMS1032

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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Your items have has been added!

Cathepsin L Monoclonal Antibody (33-2), eBioscience™

Cathepsin L Antibody (BMS1032) in WB

Product Details

BMS1032

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

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Function(s)

Process(es)

Performance Guarantee

We're here to help

Your items have has been added!