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            Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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            • Primary Antibodies ›
            • Caveolin 1 Antibodies

            Invitrogen

            Caveolin 1 Monoclonal Antibody (Z034)

            Advanced Verification
            6 Published Figures
            16 References
            View all (49) Caveolin 1 antibodies
            Datasheet
            Protocols
            Questions & Answers
            Datasheet
            Protocols
            Questions & Answers

            Cite Caveolin 1 Monoclonal Antibody (Z034)

            • Antibody Testing Data (1)
            • Published Figures (6)
            • Advanced Verification (1)
            Application
            Caveolin 1 Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 8

            Caveolin 1 Antibody (03-6000) in WB

            Knockout of Caveolin 1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR639775_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Caveolin 1 was performed by loading 30 µg of HeLa Cas9 (Lane 1), HeLa Cas9 treated with 25 nm Paclitaxel for 24 hrs (Lane 2), HeLa Caveolin 1 KO (Lane 3) and HeLa Caveolin 1 KO treated with 25 nm Paclitaxel for 24 hrs (Lane 4) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Prote... View More
            View Product

            Caveolin 1 Antibody in Western Blot (WB)
            Caveolin 1 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)
            Caveolin 1 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)
            Caveolin 1 Antibody in Immunocytochemistry (ICC/IF)
            Caveolin 1 Antibody in Immunocytochemistry (ICC/IF)
            Caveolin 1 Antibody in Immunocytochemistry (ICC/IF)
            Caveolin 1 Antibody in Western Blot (WB)
            Caveolin 1 Antibody

            Product Details

            03-6000

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            0.5-2.0 µg/mL
            View 6 publications 6 publications

            Immunohistochemistry (IHC)

            -
            View 1 publication 1 publication

            Immunohistochemistry (Frozen) (IHC (F))

            -
            View 1 publication 1 publication

            Immunocytochemistry (ICC/IF)

            2-4 µg/mL
            View 4 publications 4 publications

            Flow Cytometry (Flow)

            -
            View 1 publication 1 publication

            Immunoprecipitation (IP)

            2-5 µg/mL
            View 2 publications 2 publications

            Miscellaneous PubMed (Misc)

            -
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Human

            Published species

            Hamster, Human, Mouse, Non-human primate, Protozoa, Rabbit

            Host/Isotype

            Mouse / IgG1

            Class

            Monoclonal

            Type

            Antibody

            Clone

            Z034

            Immunogen

            Tyrosine-phosphorylated proteins from RSV-transformed chick embryo fibroblasts
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.5 mg/mL

            Purification

            purified

            Storage buffer

            PBS, pH 7.4

            Contains

            0.1% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2532932

            Target Information

            Caveolin-1 or CAV1 is a scaffolding proteins within caveolar membranes. It interacts directly with G-protein alpha subunits and can functionally regulate their activity. Caveolin-1 is also involved in the co-stimulatory signal essential for T-cell receptor (TCR) mediated T-cell activation. It can bind with DPP4 and induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Mutations affecting the CAV1 gene can result in congenital generalized lipodystrophy 3, pulmonary hypertension primary 3, or partial lipodystrophy/congenital cataracts, and neurodegeneration syndrome.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: caveolae; caveolin 1, caveolae protein, 22kDa; Caveolin-1; cell growth-inhibiting protein 32; HGNC:1527; OTTHUMP00000196475

            View more View less

            Gene Aliases: BSCL3; CAV; CAV1; CGL3; LCCNS; MSTP085; PPH3; VIP21

            View more View less

            UniProt ID: (Human) Q03135

            View more View less

            Entrez Gene ID: (Human) 857

            View more View less

            Function(s)

            RNA polymerase I transcription factor binding receptor binding patched binding structural molecule activity protein binding cholesterol binding peptidase activator activity enzyme binding protein kinase binding syntaxin binding protein binding, bridging protein complex scaffold identical protein binding ion channel binding protein heterodimerization activity Rac GTPase binding nitric-oxide synthase binding ATPase binding inward rectifier potassium channel inhibitor activity MDM2/MDM4 family protein binding scaffold/adaptor protein

            Process(es)

            negative regulation of transcription from RNA polymerase II promoter MAPK cascade inactivation of MAPK activity angiogenesis vasculogenesis response to hypoxia negative regulation of endothelial cell proliferation positive regulation of endothelial cell proliferation negative regulation of cytokine-mediated signaling pathway response to ischemia regulation of the force of heart contraction by chemical signal triglyceride metabolic process calcium ion transport cellular calcium ion homeostasis regulation of smooth muscle contraction skeletal muscle tissue development response to nutrient lactation protein localization response to bacterium response to gamma radiation positive regulation of calcium ion transport into cytosol posttranscriptional regulation of gene expression positive regulation of gene expression negative regulation of muscle cell apoptotic process positive regulation of peptidase activity vesicle organization receptor-mediated endocytosis of virus by host cell regulation of fatty acid metabolic process lipid storage regulation of blood coagulation cholesterol transport negative regulation of transforming growth factor beta receptor signaling pathway negative regulation of epithelial cell differentiation mammary gland development organ regeneration positive regulation of microtubule polymerization T cell costimulation negative regulation of protein ubiquitination positive regulation of protein ubiquitination receptor internalization negative regulation of protein binding positive regulation of protein binding maintenance of protein location in cell response to progesterone negative regulation of peptidyl-serine phosphorylation positive regulation of peptidyl-serine phosphorylation cholesterol efflux nitric oxide homeostasis positive regulation of toll-like receptor 3 signaling pathway wound healing vasoconstriction response to drug negative regulation of tyrosine phosphorylation of Stat5 protein cholesterol homeostasis positive regulation of catalytic activity negative regulation of MAPK cascade response to estrogen protein localization to plasma membrane raft negative regulation of nitric oxide biosynthetic process negative regulation of neuron differentiation positive regulation of endocytosis positive regulation of vasoconstriction negative regulation of JAK-STAT cascade microtubule polymerization negative regulation of pinocytosis negative regulation of smooth muscle cell proliferation leukocyte migration regulation of nitric-oxide synthase activity negative regulation of nitric-oxide synthase activity protein homooligomerization response to glucocorticoid regulation of cytosolic calcium ion concentration response to calcium ion membrane depolarization regulation of peptidase activity calcium ion homeostasis mammary gland involution positive regulation of cell adhesion molecule production negative regulation of necroptotic process negative regulation of protein tyrosine kinase activity negative regulation of ERK1 and ERK2 cascade caveola assembly cellular response to mechanical stimulus cellular response to exogenous dsRNA cellular response to peptide hormone stimulus cellular response to hyperoxia cellular response to transforming growth factor beta stimulus caveolin-mediated endocytosis regulation of heart rate by cardiac conduction angiotensin-activated signaling pathway involved in heart process negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway cellular senescence apoptotic signaling pathway regulation of membrane repolarization during action potential regulation of cardiac muscle cell action potential involved in regulation of contraction regulation of ventricular cardiac muscle cell action potential regulation of ruffle assembly negative regulation of peptidyl-tyrosine autophosphorylation negative regulation of potassium ion transmembrane transport regulation of cell communication by electrical coupling involved in cardiac conduction regulation of inward rectifier potassium channel activity positive regulation of ER-associated ubiquitin-dependent protein catabolic process protein localization to basolateral plasma membrane positive regulation of gap junction assembly beta-catenin destruction complex disassembly receptor internalization involved in canonical Wnt signaling pathway regulation of entry of bacterium into host cell negative regulation of anoikis positive regulation of extrinsic apoptotic signaling pathway positive regulation of intrinsic apoptotic signaling pathway negative regulation of cation channel activity
            It has to be done as per old AB suggested Products section.
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