Loading ...

Rockland

Cdc2 p34 Monoclonal Antibody (POH-1)

View all (89) CDK1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (3)

Application

FIGURE: 1 / 3

Cdc2 p34 Antibody (200-301-160) in WB

Western Blot of Mouse anti-CDC2 (p34) antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-363). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A549 Lysate (p/n W09-001-372). Lane 6: HL-60 Lysate (p/n W09-001-GL3). Lane 7: Raji Lysate (p/n W09-001-368). Lane 8: NIH/3T3 Lysate (p/n W10-000-358). Load: 35 µg per lane. Primary antibody: CDC2 (p34) antibody at 1:5,000 for overnight at 4°C. Secondary antibody: Peroxid... View More

Product Image Gallery

Product Details

200-301-160

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

ELISA (ELISA)

1:5,000-1:20,000

Immunoprecipitation (IP)

1:500-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

POH-1

Immunogen

This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.5M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Reacts specifically with p34 cdc2 in human tissues and cell lines. This antibody is not cross reactive with other cyclin dependent kinases. Cross reactivity with p34 cdc2 from other sources, especially mouse and rat will occur. This reagent has broad interspecies reactivity.

Target Information

CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: ABL1; CDK1; cell cycle controller CDC2; cell cycle p34 CDC2 kinase protein; Cell division control protein 2 homolog; cell division cycle 2 homolog A; cell division cycle 2, G1 to S and G2 to M; Cell division cycle control protein 2; cell division cycle control protein 2a; Cell division protein kinase 1; Cyclin-dependent kinase 1; DKFZp686L20222; KCIP-1; MGC111195; MGC53829; OTTHUMP00000019660; P34 CDK1; P34 MGC111195; p34 protein kinase; Protein 1054

Gene Aliases: CDC2; CDC28A; Cdc2a; CDK1; CDKN1; p34; P34CDC2

UniProt ID: (Human) P06493, (Rat) P39951, (Mouse) P11440

Entrez Gene ID: (Human) 983, (Rat) 54237, (Mouse) 12534

Function(s)

protein kinase activity protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity cyclin binding Hsp70 protein binding histone kinase activity kinase activity nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

Process(es)

G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle activation of MAPK activity microtubule cytoskeleton organization DNA replication DNA repair protein complex assembly apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest mitotic nuclear division mitotic nuclear envelope disassembly mitotic G2 DNA damage checkpoint centrosome cycle pronuclear fusion cell aging response to toxic substance positive regulation of gene expression regulation of Schwann cell differentiation response to organic cyclic compound response to amine response to activity cell migration histone phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation chromosome condensation epithelial cell differentiation organ regeneration anaphase-promoting complex-dependent catabolic process positive regulation of protein import into nucleus, translocation protein localization to kinetochore response to drug protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process response to ethanol positive regulation of DNA replication positive regulation of mitotic cell cycle regulation of embryonic development response to cadmium ion response to copper ion response to axon injury cell division negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle ventricular cardiac muscle cell development positive regulation of cardiac muscle cell proliferation cellular response to hydrogen peroxide Golgi disassembly positive regulation of protein localization to nucleus mitotic cell cycle protein phosphorylation response to organonitrogen compound response to hydrogen peroxide cell cycle phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more