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Application
FIGURE: 1 / 1
Cdc45L Antibody (PA5-96047) in WB
Western blot analysis of Cdc45L in extracts of various cell lines. Samples were incubated with Cdc45L Polyclonal antibody (Product # PA5-96047) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Product Details
PA5-96047
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CDC45 (NP_0034951)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807849
Product Specific Information
Immunogen sequence: MFVSDFRKEF YEVVQSQRVL LFVASDVDAL CACKILQALF QCDHVQYTLV PVSGWQELET AFLEHKEQFH YFILINCGAN VDLLDILQPD EDTIFFVCDT HRPVNVVNVY NDTQIKLLIK QDDDLEVPAY EDIFRDEEED EEHSGNDSDG SEPSEKRTRL EEEIVEQTMR RRQRREWEAR RRDILFDYEQ YEYHGTSSAM VMFELAWMLS KDLNDMLWWA IVGLTDQWVQ DKITQMKYVT DVGVLQRHVS RHNHRNEDEE NTLSVDCTRI; Positive Samples: U-251MG, HeLa, A-549, HL-60, HT-29, Mouse thymus; Cellular Location: Cytoplasm, Nucleus
Target Information
The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenylation sites of this gene are reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDC 45; CDC 45L; CDC45 cell division cycle 45 homolog; CDC45 like; CDC45-related protein; Cell division control protein 45 homolog; cell division cycle 45 homolog; cell division cycle 45-like 2; human CDC45; PORC PI 1; PORC-PI-1
Gene Aliases: CDC45; CDC45L; CDC45L2; PORC-PI-1; UNQ374/PRO710
UniProt ID: (Human) O75419, (Mouse) Q9Z1X9
Entrez Gene ID: (Human) 8318, (Rat) 287961, (Mouse) 12544
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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