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Invitrogen
Chromogranin B Recombinant Rabbit Monoclonal Antibody (5N6T1)
Application
FIGURE: 1 / 2
Chromogranin B Antibody (MA5-42817) in WB
Western blot analysis of Chromogranin B in extracts of various cell lines. Samples were incubated with Chromogranin B Monoclonal antibody (Product # MA5-42817) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
MA5-42817
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5N6T1
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 550-650 of human Chromogranin B (P05060).
Conjugate
Unconjugated
Form
Liquid
Concentration
1.25 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911958
Product Specific Information
Positive test controls include: 293T, PC-12, BxPC-3, SH-SY5Y, Mouse brain.
Immunogen sequence: EEENELTLNE KNFFPEYNYD WWEKKPFSED VNWGYEKRNL ARVPKLDLKR QYDRVAQLDQ LLHYRKKSAE FPDFYDSEEP VSTHQEAENE KDRADQTVLT E
Target Information
Chromogranin B is a tyrosine sulfated secretory protein found in a wide variety of peptidergic endocrine cells. Chromogranin functions as a neuroendocrine secretory granule protein which may be the precursor for other biologically active peptides.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CgB; Chromogranin B, parathyroid secretory protein; Chromogranin-B; Glucagonoma peptide; secretogranin B; Secretogranin I; Secretogranin-1; Secretogranin-I; SgI
Gene Aliases: CHGB; Scg-1; SCG1
UniProt ID: (Human) P05060, (Mouse) P16014, (Rat) O35314
Entrez Gene ID: (Human) 1114, (Mouse) 12653, (Rat) 24259
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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