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AbboMax
Cit-Histone H3 (Arg2, Arg8, Arg17) Polyclonal Antibody
FIGURE: 1 / 1
Cit-Histone H3 (Arg2, Arg8, Arg17) Antibody (630-180ABBOMAX) in ICC/IF
ImmunoFluorescent Chemistry: Serum-depleted 3T3 cells were cultured in chamber, and fixed onto slide followed by incubation with 5 µg/mL Rabbit Anti-Citrullinated Histone H3 (R2+R8+R17) antibody, (Cat# 630-180), followed by then Rhodamine labeled Goat anti Rabbit IgG for visualization under fluorescent microscope.
Product Details
630-180ABBOMAX
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Chicken,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide containing N-terminus of human histone H3 with citrulline modification at 2/8/17
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with proprietary stabilizer
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Positive control: 3T3 Nuclear Extract
Cellular location: Nuclear.
Target Information
Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The methylation of specific residues in the histone tails is a central modification for regulatingepigenetic transitions in chromatin. Whereas methylation of histone H3 on lysine 4, 36, and 79 has been linked with gene activation, methylation of H3 on lysines 9, 27 and histone H4 on lysine 20 is associated with heterochromatin and some repressed genes with euchromatin. Modified lysine residues can exist in a mono-, di-, or tri-methylated state, while the arginine residues can be mono- or di-methylated. Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin. This antibody is specific for histone H3 tri-methylated at K27. The sequence is found in all mammals and a wide range of species, including D. melanogaster, Arabidopsis, Chicken and Xenopus. The antibody will react with any of the above species where the trimethylation modification is present.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Citrullinated Histone H3; H3 citrulline (R2/8/17); H3 histone family, member A; H3 histone family, member B; H3 histone family, member C; H3 histone family, member D; H3 histone family, member F; H3 histone family, member H; H3 histone family, member I; H3 histone family, member K; H3 histone family, member L; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; histone 1, H3a; histone 1, H3b; histone 1, H3c; histone 1, H3d; histone 1, H3e; histone 1, H3f; histone 1, H3g; histone 1, H3h; histone 1, H3i; histone gene complex 1; histone H3; histone H3 with citrulline modification at 2/8/17; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; histone1, H3c; histone1, H3d
Gene Aliases: H3-143; H3-291; H3-53; H3-B; H3-F; H3.1; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3.f; H3/A; H3/b; H3/c; H3/d; H3/f; H3/h; H3/i; H3/k; H3/l; H3a; H3b; H3C1; H3C10; H3C11; H3C12; H3c13; H3c14; H3c15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F1K; H3FA; H3FB; H3FC; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3g; H3h; H3i; Hist1; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; Hist2h3b; Hist2h3c1; Hist2h3c2; Hist2h3ca1; Hist2h3ca2; M32460
UniProt ID: (Human) P68431, (Human) Q6NXU4, (Mouse) P68433, (Mouse) P84228
chromatin silencing at rDNA
positive regulation of defense response to virus by host
nucleosome assembly
DNA replication-dependent nucleosome assembly
blood coagulation
gene silencing by RNA
telomere organization
cellular protein metabolic process
negative regulation of gene expression, epigenetic
positive regulation of gene expression, epigenetic
protein heterotetramerization
regulation of gene silencing
cell-cell adhesion
xenophagy
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