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            • Primary Antibodies ›
            • Claudin 3 Antibodies

            Invitrogen

            Claudin 3 Polyclonal Antibody

            Advanced Verification
            88 Published Figures
            279 References
            View all (23) Claudin 3 antibodies
            Datasheet
            Protocols
            Questions & Answers
            Catalog # 34-1700
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            Price (USD)
            488.00
            100 µg
             
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            Datasheet
            Protocols
            Questions & Answers

            Cite Claudin 3 Polyclonal Antibody

            • Antibody Testing Data (4)
            • Published Figures (88)
            • Advanced Verification (1)
            Application
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 93

            Claudin 3 Antibody (34-1700) in ICC/IF

            Immunofluorescence analysis of Claudin-3 was performed using 70% confluent log phase NIH:OVCAR-3 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Claudin 3 Polyclonal Antibody (Product # 34-1700) at 1:100 dilution in 0.1% BSA, incubated at 4 degree ... View More
            View Product

            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
            Claudin 3 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
            Claudin 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Claudin 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Claudin 3 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
            Claudin 3 Antibody in Immunohistochemistry (Frozen) (IHC (F))
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
            Claudin 3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
            Claudin 3 Antibody in Immunohistochemistry (IHC)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody in Western Blot (WB)
            Claudin 3 Antibody

            Product Details

            34-1700

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:1,000
            View 109 publications 109 publications

            Immunohistochemistry (IHC)

            -
            View 64 publications 64 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            1:50-1:200
            View 12 publications 12 publications

            Immunohistochemistry (Frozen) (IHC (F))

            -
            View 10 publications 10 publications

            Immunocytochemistry (ICC/IF)

            1:100
            View 69 publications 69 publications

            ELISA (ELISA)

            Assay-dependent
            -

            Immunoprecipitation (IP)

            -
            View 2 publications 2 publications

            in situ PLA (PLA)

            -
            View 1 publication 1 publication

            Immunoelectrophoresis (IE)

            -
            View 1 publication 1 publication

            Miscellaneous PubMed (Misc)

            -
            View 11 publications 11 publications
            Product Specifications

            Species Reactivity

            Dog, Human, Mouse

            Published species

            Amphibian, Bovine, Dog, Fish, Goat, Hamster, Human, Mouse, Pig, Plant, Rabbit, Rat, Rhesus monkey, Sheep, Virus, Xenopus, Zebrafish

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Synthetic peptide derived from the C-terminal region of mouse Claudin-3
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.25 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS, pH 7.4

            Contains

            0.1% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2533158

            Product Specific Information

            This antibody is specific for the 22 kDa Claudin-3 protein. Reactivity has been confirmed with mouse liver and lung homogenates, canine MDCK and human MCF-7 cell lysates by western blotting, and with formalin-fixed, paraffin-embedded (FFPE) human normal colon and colon cancer tissues by immunohistochemistry. For best results in immunohistochemistry with formalin-fixed, paraffin-embedded (FFPE) tissues, heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining.

            Target Information

            Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc. Alternatively spliced transcript variants encoding different isoforms have been found.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Related Products for

            Bioinformatics

            Protein Aliases: Claudin-3; Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-receptor 2; hRVP1; integral membrane protein claudin-3; Rat ventral prostate.1 protein homolog; ventral prostate.1 protein homolog; ventral prostate.1-like protein

            View more View less

            Gene Aliases: AI182374; C7orf1; CLDN3; CPE-R2; CPETR2; HRVP1; mRVP1; RVP1

            View more View less

            UniProt ID: (Human) O15551, (Dog) Q95KM5, (Mouse) Q9Z0G9

            View more View less

            Entrez Gene ID: (Human) 1365, (Dog) 403648, (Mouse) 12739

            View more View less

            Function(s)

            transmembrane signaling receptor activity structural molecule activity identical protein binding protein binding tight junction

            Process(es)

            response to hypoxia epithelial cell morphogenesis signal transduction calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules protein homooligomerization protein heterooligomerization bicellular tight junction assembly positive regulation of protein phosphorylation negative regulation of cell proliferation positive regulation of gene expression negative regulation of gene expression establishment of endothelial blood-brain barrier regulation of cell morphogenesis positive regulation of cell migration negative regulation of cell migration actin cytoskeleton reorganization cell junction maintenance negative regulation of wound healing positive regulation of wound healing regulation of membrane permeability positive regulation of bicellular tight junction assembly positive regulation of metallopeptidase activity
            It has to be done as per old AB suggested Products section.
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