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Proteintech

Cleaved Caspase 3 Recombinant Rabbit Monoclonal Antibody (230527B2)

View all (2) Caspase 3 p17/p19 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)

Application

Cleaved Caspase 3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

Cleaved Caspase 3 Antibody (82707-13-RR) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cleaved Caspase 3 antibody (82707-13-RR, Clone: 230527B2) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (SA00013-1).

Cleaved Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cleaved Caspase 3 Antibody in Western Blot (WB)

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Product Details

82707-13-RR

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

Immunocytochemistry (ICC/IF)

1:125-1:500

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

CHO cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

230527B2

Immunogen

Peptide

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Target Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. The cleaved fragment of Caspase 3 might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Apopain; CASP-3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; CPP-32; Cysteine protease CPP32; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1

Gene Aliases: CASP3; CPP32; CPP32B; SCA-1

UniProt ID: (Human) P42574

Entrez Gene ID: (Human) 836

Function(s)

protease binding aspartic-type endopeptidase activity cysteine-type endopeptidase activity cyclin-dependent protein serine/threonine kinase inhibitor activity death receptor binding protein binding peptidase activity cysteine-type endopeptidase activator activity involved in apoptotic process phospholipase A2 activator activity protein complex binding cysteine-type endopeptidase activity involved in apoptotic process cysteine-type endopeptidase activity involved in execution phase of apoptosis protease

Process(es)

response to hypoxia B cell homeostasis apoptotic DNA fragmentation proteolysis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cellular component disassembly involved in execution phase of apoptosis cellular response to DNA damage stimulus heart development sensory perception of sound learning or memory activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c response to UV response to glucose response to X-ray regulation of macroautophagy protein processing hippocampus development neuron differentiation keratinocyte differentiation erythrocyte differentiation platelet formation negative regulation of B cell proliferation response to cobalt ion response to estradiol response to lipopolysaccharide glial cell apoptotic process response to tumor necrosis factor response to nicotine hippo signaling wound healing response to drug response to hydrogen peroxide T cell homeostasis negative regulation of apoptotic process response to amino acid positive regulation of neuron apoptotic process cell fate commitment negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of activated T cell proliferation response to antibiotic neurotrophin TRK receptor signaling pathway response to glucocorticoid neuron apoptotic process cellular response to organic cyclic compound apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand execution phase of apoptosis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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