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Invitrogen
Clostridium difficile GDH Monoclonal Antibody (5194)
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Product Details
MA5-47559
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
5194
Immunogen
Proprietary sequence
Conjugate
Unconjugated
Form
Liquid
Concentration
100 µg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.1% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2942540
Product Specific Information
Specificity: Specific for glutamate dehydrogenase of C. difficile. Non-reactive with mammalian cells.
Best ELISA pairs (capture/conjugate): Clostridium difficile GDH Monoclonal antibody (Product # MA5-47559)/Clostridium difficile GDH Monoclonal antibody (Product # MA5-47561).
Purified preparations consist of >90% pure mouse monoclonal antibody which has been purified from ascites fluid by protein A chromatography. The final preparation is formulated to a protein concentration of 100 µg/mL in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide. Each vial contains 1.0 mL. This product contains no stabilizing proteins and should be stored at 4C until ready for use. Working dilution must be determined by the user. Suggested starting ranges are 1:10-1:50 for ICC/IF and 1:20-1:200. for ELISA.
Target Information
Clostridium difficile produces an NAD-specific glutamate dehydrogenase (GDH), which converts l-glutamate into alpha-ketoglutarate through an irreversible reaction. The enzyme GDH is detected in the stool samples of patients with C. difficile-associated disease and serves as one of the diagnostic tools to detect C. difficile infection (CDI). [PMID: 24145018]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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