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Invitrogen
Cofilin Monoclonal Antibody (GT567)
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Application
FIGURE: 1 / 9
Cofilin Antibody (MA5-17275) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Cofilin was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 10 min. Green: Cofilin Monoclonal Antibody (GT567) (Product # MA5-17275) diluted at 1:300. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
MA5-17275
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
GT567
Immunogen
Recombinant protein encompassing a sequence within the center region of human Cofilin 1. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2538741
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, A375, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat-2.
Predicted reactivity: Mouse (98%), Rat (99%), Pig (99%), Sheep (99%), Rhesus Monkey (99%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Cofilin binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 18 kDa phosphoprotein; CFL-1; COF1; cofilin 1 (non-muscle); Cofilin, non-muscle isoform; Cofilin-1; Cofilin1; epididymis secretory protein Li 15; p18
Gene Aliases: AA959946; CFL; CFL1; Cof; cofilin; HEL-S-15; n-cofilin
UniProt ID: (Human) P23528, (Rat) P45592, (Mouse) P18760
Entrez Gene ID: (Human) 1072, (Rat) 29271, (Mouse) 12631
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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