Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
NeoBiotechnologies
Complement 3d (C3d) (Acute Humoral Rejection Marker) Monoclonal Antibody (C3D/2891)
Application
FIGURE: 1 / 4
Complement 3d (C3d) (Acute Humoral Rejection Marker) Antibody (718-MSM1-P1) in IHC (P)
Formalin-fixed, paraffin-embedded human rejected kidney transplant stained with Complement 3d Mouse Monoclonal Antibody (C3D/2891) at 2 µg/mL in PBS. HIER: Tris/EDTA, pH9.0, 45 min. 2nd Ab: HRP-polymer, 30 min. DAB, 5 min.
Product Details
718-MSM1-P1
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
C3D/2891
Immunogen
Recombinant fragment (around aa 1250-1450) of human C3 protein.
Conjugate
Unconjugated
Form
Liquid
Concentration
200 µg/mL
Purification
Protein A/G
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Antibody is stable for 24 months.
Positive Control: Human liver cancer or rejected renal transplant tissue. Cellular Localization: Plasma.
Specificity Comments: The complement component proteins, C2, C3, C4 and C5, are potent anaphylatoxins that are released during complement activation. Binding of these proteins to their respective G protein-coupled receptors, C3aR, C1R and C5aR, induces proinflammatory events, such as cellular degranulation, smooth muscle contraction, arachidonic acid metabolism, cytokine release, leukocyte activation and cellular chemotaxis. C3d is a terminal degradation product of C3 that plays an important role in modulation of the adaptive immune response through the interaction with complement receptor type 2 (CR2). CR2 is important in the switched-isotype, high-affinity and memory humoral immune responses to T-dependent foreign antigens, as well as in the development of the natural antibody repertoire. This pH- and ionic strength-dependent association of C3d with CR2 represents a link between innate and adaptive immunity.
Target Information
C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of two chains (110 kDa and 75 kDa) held together by disulfide bonds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Acylation stimulating protein cleavage product; acylation-stimulating protein cleavage product; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Cd3; chain fragment 2; Complement C3; Complement C3c alpha' complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; prepro-C3
Gene Aliases: AHUS5; ARMD9; ASP; C3; C3a; C3b; CPAMD1; HEL-S-62p
UniProt ID: (Human) P01024
Entrez Gene ID: (Human) 718
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support