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Bioss

Connexin-40 Polyclonal Antibody

View all (9) Connexin 40 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)

Application

Connexin-40 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 2

Connexin-40 Antibody (BS-1050R) in IHC (P)

Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Connexin 40 Polyclonal Antibody, Unconjugated (bs-1050R) at 1:500 overnight at 4°C, followed by a conjugated ... View More

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Product Details

BS-1050R

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:500

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:50-1:200

Flow Cytometry (Flow)

1:20-1:100

ELISA (ELISA)

1:500-1:1,000

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human Connexin-40, amino acids 301-358.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Connexin 40 (Cx40), belongs to the connexin family of gap junction proteins, which are expressed in pancreas, kidney, skeletal muscle, liver, placenta and heart. Connexin 40 is highly expressed in vascular endothelial cells, and the mammalian heart (specifically in the atrial myocardium, and the His-Purkinje conduction system). At least three other connexins are expressed in the heart, including Cx37 (endocardium), Cx43 (atrial and ventricular myocardium, and distal parts of the conduction system) and Cx45 (throughout the heart). Cx40 and Cx43 frequently co-localize in cells of the cardiovascular system and participate in the formation of heterotypic gap junction channels.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: connexin 40; Connexin-40; Cx40; Gap junction alpha-5 protein; gap junction membrane channel protein alpha 5; gap junction protein; gap junction protein alpha 5 40kDa

Gene Aliases: 5730555N10Rik; ATFB11; Cnx40; CX40; Cxn-40; Gja-5; GJA5

UniProt ID: (Dog) P33725, (Human) Q5U0N6, (Rat) P28234, (Mouse) Q01231

Entrez Gene ID: (Dog) 483155, (Human) 2702, (Rat) 50563, (Mouse) 14613

Function(s)

gap junction hemi-channel activity gap junction channel activity involved in atrial cardiac muscle cell-AV node cell electrical coupling gap junction channel activity involved in AV node cell-bundle of His cell electrical coupling gap junction channel activity involved in bundle of His cell-Purkinje myocyte electrical coupling connexin binding gap junction channel activity involved in cardiac conduction electrical coupling gap junction channel activity gap junction

Process(es)

skeletal system development angiogenesis renal system process involved in regulation of systemic arterial blood pressure outflow tract morphogenesis cardiac conduction system development mitral valve development pulmonary valve formation septum primum development atrial ventricular junction remodeling gap junction assembly embryonic limb morphogenesis embryonic heart tube development foramen ovale closure artery morphogenesis transmembrane transport regulation of ventricular cardiac muscle cell membrane repolarization regulation of atrial cardiac muscle cell membrane depolarization regulation of ventricular cardiac muscle cell membrane depolarization ventricular septum morphogenesis atrial septum morphogenesis ventricular cardiac muscle cell action potential SA node cell action potential atrial cardiac muscle cell to AV node cell communication by electrical coupling AV node cell to bundle of His cell communication by electrical coupling bundle of His cell to Purkinje myocyte communication by electrical coupling regulation of heart rate by cardiac conduction regulation of blood vessel diameter regulation of AV node cell action potential regulation of bundle of His cell action potential regulation of Purkinje myocyte action potential regulation of atrial cardiac muscle cell action potential regulation of renin secretion into blood stream regulation of membrane depolarization during cardiac muscle cell action potential negative regulation of glomerular filtration endothelium development ventricular septum development atrial septum development potassium ion transport cell communication by chemical coupling positive regulation of cell communication by chemical coupling negative regulation of blood pressure positive regulation of vasoconstriction positive regulation of vasodilation protein oligomerization regulation of cardiac muscle contraction cell communication by electrical coupling involved in cardiac conduction vasomotion blood vessel development regulation of systemic arterial blood pressure heart development cell communication by electrical coupling regulation of vasodilation cardiac conduction atrial cardiac muscle cell action potential cell communication involved in cardiac conduction AV node cell to bundle of His cell communication bundle of His cell to Purkinje myocyte communication cell communication

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