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QED Bioscience

Copper-Transporting ATPase1 Monoclonal Antibody (S60-4)

View all (16) ATP7A antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)

Application

Copper-Transporting ATPase1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

Copper-Transporting ATPase1 Antibody (56516-100UG) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody, Clone S60-4 (56516). Tissue: NIH 3T3 (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody (56516) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Co... View More

Copper-Transporting ATPase1 Antibody in Western Blot (WB)

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Product Details

56516-100UG

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

S60-4

Immunogen

Synthetic peptide correspond-ing to aa 42-61 (cytoplasmic C-terminus) of human Copper-Transporting ATPase1 (accession no. NP_000043.3).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

ATP7A (also known as Copper-transporting ATPase 1) functions as a transmembrane copper-translocating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Polarized epithelial cells such as Madin-Darby canine kidney cells are a physiologically relevant model for systemic copper absorption and reabsorption in vivo. Although ATP7A is not detectable in most normal tissues, it is expressed in a considerable fraction of many common tumor types. Increased expression of ATP7A renders cells resistant to cisplatin and carboplatin. Mutations in the ATP7A gene result in Menkes disease, which is fatal in early childhood.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP 7A; ATPase, Cu++ transporting, alpha polypeptide; Copper pump 1; Copper-transporting ATPase 1; Cu++-transporting P-type ATPase; FLJ17790; MC 1; Menke; Menkes disease-associated protein; Menkes disease-associated protein homolog; Menkes protein; Menkes syndrome; OHS; OTTHUMP00000062077

Gene Aliases: ATP7A; DSMAX; MC1; MK; MNK; SMAX3

UniProt ID: (Human) Q04656, (Mouse) Q64430, (Rat) P70705

Entrez Gene ID: (Human) 538, (Mouse) 11977, (Rat) 24941

Function(s)

copper-exporting ATPase activity copper ion transmembrane transporter activity copper ion binding protein binding ATP binding superoxide dismutase copper chaperone activity copper-dependent protein binding nucleotide binding hydrolase activity cation-transporting ATPase activity metal ion binding molecular_function primary active transporter

Process(es)

blood vessel development in utero embryonic development blood vessel remodeling regulation of oxidative phosphorylation tryptophan metabolic process catecholamine metabolic process copper ion transport cellular copper ion homeostasis mitochondrion organization lactation locomotory behavior response to iron(III) ion response to zinc ion detoxification of copper ion copper ion import plasma membrane copper ion transport peptidyl-lysine modification removal of superoxide radicals cerebellar Purkinje cell differentiation pyramidal neuron development central nervous system neuron development extracellular matrix organization collagen fibril organization hair follicle morphogenesis ion transmembrane transport T-helper cell differentiation epinephrine metabolic process norepinephrine metabolic process dopamine metabolic process serotonin metabolic process positive regulation of catalytic activity negative regulation of catalytic activity pigmentation skin development elastic fiber assembly lung alveolus development neuron projection morphogenesis cartilage development positive regulation of oxidoreductase activity elastin biosynthetic process copper ion export release of cytochrome c from mitochondria tyrosine metabolic process transport ion transport cation transport regulation of gene expression metal ion transport hindlimb morphogenesis norepinephrine biosynthetic process negative regulation of neuron apoptotic process ATP metabolic process response to copper ion dendrite morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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