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Invitrogen
Cre recombinase Polyclonal Antibody
Application
FIGURE: 1 / 3
Cre recombinase Antibody (PA5-32244) in WB
Cre recombinase Polyclonal Antibody detects Cre recombinase protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of Cre-ERT2-transfected 293T cells (Cre-ERT2 fusion protein size: 72 kDa).7.5 % SDS-PAGE. Cre recombinase Polyclonal Antibody (Product # PA5-32244) dilution: 1:10,000.
Product Details
PA5-32244
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of Cre recombinase. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
1.27 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2549717
Product Specific Information
Recommended positive controls: 293T transfected recombinant cre protein, IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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