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            Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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            • Primary Antibodies ›
            • Cripto Antibodies

            Rockland

            Cripto-1 TDGF1 Polyclonal Antibody

            View all (20) Cripto antibodies
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            Cite Cripto-1 TDGF1 Polyclonal Antibody

            • Antibody Testing Data (1)
            Application
            Cripto-1 TDGF1 Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 1

            Cripto-1 TDGF1 Antibody (600-401-997) in WB

            Western blot using Rocklands affinity purified anti-Cripto-1 antibody shows detection of endogenous and transfected Cripto-1 from mouse and human sources. The Cripto-1 band appears above the 17.8 kDa marker. Endogenous detection is shown using mouse P19 embryonal carcinoma cells and human NCCIT testicular embryonal carcinoma cells. EpH4 CR-1 is a mouse mammary epithelial cell line stably expressing mouse Cripto-1. NTERA cells are human embryonal carcinoma cells that, when overgrown, differentiate and lose Cripto-1 expression. COS7 cells tr... View More
            View Product

            Cripto-1 TDGF1 Antibody in Western Blot (WB)

            Product Details

            600-401-997

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:1,000
            -

            Immunohistochemistry (IHC)

            Assay-dependent
            -

            ELISA (ELISA)

            1:105,000
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human Cripto-1 protein.

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.97 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

            Contains

            0.01% sodium azide

            Storage conditions

            -20°C, Avoid Freeze/Thaw Cycles

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

            This antibody is specific for human Cripto-1 protein. A BLAST analysis was used to suggest limited cross-reactivity with Cripto-1 from mouse based on a 70% homology with the immunizing sequence. Expect cross-reactivity with human Cripto-3 (TDGF2) based on very high levels of sequence conservation. Cross-reactivity with Cripto-1 from other sources has not been determined.

            Target Information

            Cripto (TDGF1) is a GPI-anchored cell membrane proetin involved in Nodal signaling. Cell associated TDGF1 acts as a a Nodeal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulated Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells. TDGF1 is expressed both in ES cells and during the early phases of embryo development, while in adults it is reactivated in a wide range of epithelial cancers.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: CRGF; Cripto, EGF-CFC family member; Cripto-1; Cripto-1 growth factor; Cripto-3; Epidermal growth factor-like cripto protein CR1; Protein Cripto; TDGF2; Teratocarcinoma-derived growth factor 1

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            Gene Aliases: CR; CRGF; CRIPTO; CRIPTO-1; TDGF1

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            UniProt ID: (Human) P13385

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            Entrez Gene ID: (Human) 6997

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            Function(s)

            receptor binding protein binding growth factor activity intercellular signal molecule

            Process(es)

            activation of MAPK activity morphogenesis of a branching structure cell migration involved in sprouting angiogenesis epidermal growth factor receptor signaling pathway heart development positive regulation of cell proliferation anterior/posterior axis specification, embryo embryo development regulation of signal transduction positive regulation of endothelial cell migration peptidyl-serine phosphorylation cell differentiation positive regulation of cell migration mammary gland development somatic stem cell population maintenance cellular response to hepatocyte growth factor stimulus negative regulation of apoptotic process cellular response to fibroblast growth factor stimulus positive regulation of peptidyl-tyrosine phosphorylation cellular response to interferon-gamma cellular response to interleukin-6 cellular response to tumor necrosis factor cellular response to epidermal growth factor stimulus
            It has to be done as per old AB suggested Products section.
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            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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