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Bethyl Laboratories
Cul7 Polyclonal Antibody
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Application
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Cul7 Antibody (A300-224A) in WB
Detection of human Cul7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP) from U2OS cells. Antibodies: Affinity purified rabbit anti-Cul7 antibody BL654 (Product # A300-224A) at 1 and 5 µg/mg lysate for IP. Cul-7 was also immunoprecipitated using rabbit anti-Cul7 antibody BL653 (Product #A300-223A). Cul7 was detected by WB using BL653 at 1 µg/mL. immunoprecipitation was also performed using normal rabbit IgG (control IP) Detection: Chemiluminescence with exposure times of 10 seconds.
Product Details
A300-224A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residues 1650 and the C-terminus (residue 1698) of human Cullin homolog 7.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat
Target Information
The protein encoded by this gene is a component of an E3 ubiquitin-protein ligase complex. The encoded protein interacts with TP53, CUL9, and FBXW8 proteins. Defects in this gene are a cause of 3M syndrome type 1 (3M1). Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CUL-7; Cullin-7
Gene Aliases: 3M1; CUL7; dJ20C7.5; KIAA0076
UniProt ID: (Human) Q14999
Entrez Gene ID: (Human) 9820
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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