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Invitrogen

Cullin 3 Polyclonal Antibody

Advanced Verification

2 Published Figures

2 References

View all (16) Cullin 3 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Application

FIGURE: 1 / 5

Cullin 3 Antibody (PA5-17397) in WB

Western blot was performed using Anti-Cullin 3 Polyclonal Antibody (Product # PA5-17397) and a 81 kDa band corresponding to CUL3 was observed across cell lines and tissues tested. (Fig. a) Whole cell extracts (30ug) of HeLa (Lane 1), PC-12 (Lane 2), Neuro-2a (Lane 3), L6 (Lane 4), U-2 OS (Lane 5), Hep G2 (Lane 6), MDA-MB-231 (Lane 7), MCF 10A (Lane 8) and T-47D (Lane 9), (Fig.b) tissue extracts (30ug) of Mouse Brain (Lane 1), Rat Liver (Lane 2), Mouse Liver (Lane 3), Rat Ovary (Lane 4) and Mouse Ovary (Lane 5) were electrophoresed using No... View More

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Product Details

PA5-17397

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues surrounding Leu750 of human Cullin-3

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

32 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10985263

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Cullin 3 is a member of the family of human cullin genes (CUL 1, 2, 3, 4a, 4b, and 5) homologous to the S. cerevisiae cdc 53 gene. CUL 1 forms a complex with human p19 skp1 and F box protein p45 skp2. Together with cdc 53 (Known as Cul A), as three subunits, they form a ubiquitin ligase-controlling proteolysis of G1 cell cycle regulatory proteins. In contrast to CUL 1, CUL 2 does not associate with p19 skp1 and p45 skp2. Instead, CUL 2 forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongain C, Elongin B and VHL. The function of this association remains unknown.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NEDDylated Cullin 3 mediates the adaptive response to topoisomerase 1 inhibitors.

Science advances

Meroni A,Grosser J,Agashe S,Ramakrishnan N,Jackson J,Verma P,Baranello L,Vindigni A

Published figure using Cullin 3 polyclonal antibody (Product # PA5-17397) in Western Blot

Fri Dec 09 00:00:00 UTC 2022

The E3 Ubiquitin-Protein Ligase Cullin 3 Regulates HIV-1 Transcription.

Cells

Langer S,Yin X,Diaz A,Portillo AJ,Gordon DE,Rogers UH,Marlett JM,Krogan NJ,Young JAT,Pache L,Chanda SK

PA5-17397 was used in Western Blotting to indicate that Cul3 is a negative regulator of HIV-1 transcription which governs productive viral replication in infected cells.

Tue Sep 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: CUL-3; Cullin-3; OTTHUMP00000203839; OTTHUMP00000203840

Gene Aliases: AI467304; AW146203; CUL-3; CUL3; KIAA0617; mKIAA0617; PHA2E

UniProt ID: (Human) Q13618, (Mouse) Q9JLV5

Entrez Gene ID: (Human) 8452, (Mouse) 26554, (Rat) 301555

Function(s)

ubiquitin-protein transferase activity protein binding cyclin binding POZ domain binding ubiquitin protein ligase binding protein homodimerization activity protein heterodimerization activity ubiquitin protein ligase activity molecular_function ubiquitin-protein ligase

Process(es)

G1/S transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter MAPK cascade protein polyubiquitination trophectodermal cellular morphogenesis protein monoubiquitination ER to Golgi vesicle-mediated transport cell cycle arrest mitotic metaphase plate congression integrin-mediated signaling pathway gastrulation negative regulation of cyclin-dependent protein serine/threonine kinase by cyclin degradation positive regulation of cell proliferation Wnt signaling pathway cell migration protein ubiquitination stem cell division cell projection organization positive regulation of cytokinesis negative regulation of Rho protein signal transduction embryonic cleavage protein ubiquitination involved in ubiquitin-dependent protein catabolic process stress fiber assembly proteasome-mediated ubiquitin-dependent protein catabolic process fibroblast apoptotic process positive regulation of mitotic metaphase/anaphase transition COPII vesicle coating liver morphogenesis negative regulation of canonical Wnt signaling pathway intrinsic apoptotic signaling pathway mitotic cell cycle cell morphogenesis in utero embryonic development regulation of transcription from RNA polymerase II promoter ubiquitin-dependent protein catabolic process transport vesicle-mediated transport biological_process

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