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Invitrogen

Cyclin B1 Monoclonal Antibody (V152)

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6 Published Figures

19 References

View all (57) Cyclin B1 antibodies

Datasheet

Protocols

Datasheet

Protocols

Application

Cyclin B1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

Cyclin B1 Antibody (MA5-13128) in ICC/IF

Immunofluorescence analysis of Cyclin B1 was performed using 70% confluent log phase HT-29 cells treated 100 ng of Nocodazole for 16 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cyclin B1 Mouse (V152) Monoclonal Antibody (Product # MA5-13128) at 2µ... View More

Cyclin B1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cyclin B1 Antibody in Immunohistochemistry (IHC)

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Product Details

MA5-13128

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

2 µg/mL

Flow Cytometry (Flow)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

V152

Immunogen

Hamster cyclin B1 protein

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10988707

Product Specific Information

MA5-13128 targets Cyclin B1 in FACS, IHC (P), and WB applications and shows reactivity with Hamster and Human samples.

The MA5-13128 immunogen is hamster cyclin B1 protein.

Target Information

Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The most abundant maternal lncRNA Sirena1 acts post-transcriptionally and impacts mitochondrial distribution.

Nucleic acids research

Ganesh S,Horvat F,Drutovic D,Efenberkova M,Pinkas D,Jindrova A,Pasulka J,Iyyappan R,Malik R,Susor A,Vlahovicek K,Solc P,Svoboda P

Published figure using Cyclin B1 monoclonal antibody (Product # MA5-13128) in Western Blot

Mon Apr 06 00:00:00 UTC 2020

Menadione reduces CDC25B expression and promotes tumor shrinkage in gastric cancer.

Therapeutic advances in gastroenterology

Bona AB,Calcagno DQ,Ribeiro HF,Muniz JAPC,Pinto GR,Rocha CAM,Lacreta Junior ACC,de Assumpção PP,Herranz JAR,Burbano RR

Published figure using Cyclin B1 monoclonal antibody (Product # MA5-13128) in Western Blot

Sat Apr 09 00:00:00 UTC 2022

Targeted inhibition of metastatic melanoma through interference with Pin1-FOXM1 signaling.

Oncogene

Kruiswijk F,Hasenfuss SC,Sivapatham R,Baar MP,Putavet D,Naipal KA,van den Broek NJ,Kruit W,van der Spek PJ,van Gent DC,Brenkman AB,Campisi J,Burgering BM,Hoeijmakers JH,de Keizer PL

Published figure using Cyclin B1 monoclonal antibody (Product # MA5-13128) in Immunofluorescence

Thu Apr 28 00:00:00 UTC 2016

Targeted inhibition of metastatic melanoma through interference with Pin1-FOXM1 signaling.

Oncogene

Kruiswijk F,Hasenfuss SC,Sivapatham R,Baar MP,Putavet D,Naipal KA,van den Broek NJ,Kruit W,van der Spek PJ,van Gent DC,Brenkman AB,Campisi J,Burgering BM,Hoeijmakers JH,de Keizer PL

Published figure using Cyclin B1 monoclonal antibody (Product # MA5-13128) in Immunofluorescence

Thu Apr 28 00:00:00 UTC 2016

Aurora kinase A is not involved in CPEB1 phosphorylation and cyclin B1 mRNA polyadenylation during meiotic maturation of porcine oocytes.

PloS one

Komrskova P,Susor A,Malik R,Prochazkova B,Liskova L,Supolikova J,Hladky S,Kubelka M

MA5-13128 was used in western blot to determine meiotic maturation of porcine oocytes and how aurora kinase A is not involved in CPEB1 phosphorylation and cyclin B1 mRNA polyadenylation

Tue Nov 03 00:00:00 UTC 2015

Cellular distributions of molecules with altered expression specific to thyroid proliferative lesions developing in a rat thyroid carcinogenesis model.

Cancer science

Woo GH,Takahashi M,Inoue K,Fujimoto H,Igarashi K,Kanno J,Hirose M,Nishikawa A,Shibutani M

MA5-13128 was used in immunohistochemistry to identify molecules whose expression is altered in thyroid lesions in a rat model of thyroid carconogenesis

Wed Apr 01 00:00:00 UTC 2009

Antigenic profiling of glioma cells to generate allogeneic vaccines or dendritic cell-based therapeutics.

Clinical cancer research : an official journal of the American Association for Cancer Research

Zhang JG,Eguchi J,Kruse CA,Gomez GG,Fakhrai H,Schroter S,Ma W,Hoa N,Minev B,Delgado C,Wepsic HT,Okada H,Jadus MR

MA5-13128 was used in flow cytometry to examine the allogenic glioma cells for the generation of therapeutic vaccines or cellular therapy

Mon Jan 15 00:00:00 UTC 2007

Cyclin B1 is commonly expressed in the cytoplasm of primary human acute myelogenous leukemia cells and serves as a leukemia-associated antigen associated with autoantibody response in a subset of patients.

European journal of haematology

Ersvaer E,Zhang JY,McCormack E,Olsnes A,Anensen N,Tan EM,Gjertsen BT,Bruserud O

MA5-13128 was used in western blot to investigate the cyclin B1 expression in primary human acute myelogenous leukemia cells

Sat Sep 01 00:00:00 UTC 2007

Cdc6 stability is regulated by the Huwe1 ubiquitin ligase after DNA damage.

Molecular biology of the cell

Hall JR,Kow E,Nevis KR,Lu CK,Luce KS,Zhong Q,Cook JG

MA5-13128 was used in western blot to study the role of Huwe1 in regulating cdc6 stability after cell damage

Sat Sep 01 00:00:00 UTC 2007

The cyclin-dependent kinase inhibitor seliciclib (R-roscovitine; CYC202) decreases the expression of mitotic control genes and prevents entry into mitosis.

Cell cycle (Georgetown, Tex.)

Whittaker SR,Te Poele RH,Chan F,Linardopoulos S,Walton MI,Garrett MD,Workman P

MA5-13128 was used in western blot to study the effects of the CDK inhibitor seliciclib on the control of entry into mitosis using cDNA microarrays to assess gene expression

Sat Dec 15 00:00:00 UTC 2007

HPV16 E1--E4 protein is phosphorylated by Cdk2/cyclin A and relocalizes this complex to the cytoplasm.

Virology

Davy CE,Ayub M,Jackson DJ,Das P,McIntosh P,Doorbar J

MA5-13128 was used in Western Blotting to show that 16E1--E4 is able to associate with cyclin A and Cdk2 during the G2 phase of the cell cycle.

Thu May 25 00:00:00 UTC 2006

In vitro and in vivo pharmacokinetic-pharmacodynamic relationships for the trisubstituted aminopurine cyclin-dependent kinase inhibitors olomoucine, bohemine and CYC202.

Clinical cancer research : an official journal of the American Association for Cancer Research

Raynaud FI,Whittaker SR,Fischer PM,McClue S,Walton MI,Barrie SE,Garrett MD,Rogers P,Clarke SJ,Kelland LR,Valenti M,Brunton L,Eccles S,Lane DP,Workman P

MA5-13128 was used in western blot to study the pharmacokinetics and pharmacodynamics of three tri-substituted aminopurine Cdk inhibitors in a model of colon carcinoma

Fri Jul 01 00:00:00 UTC 2005

Prognostic significance of expressions of cell-cycle regulatory proteins in gastrointestinal stromal tumor and the relevance of the risk grade.

Human pathology

Nakamura N,Yamamoto H,Yao T,Oda Y,Nishiyama K,Imamura M,Yamada T,Nawata H,Tsuneyoshi M

MA5-13128 was used in immunohistochemistry to study the prognostic value of the expression of a range of cell cycle regulatory proteins in gastrointestinal stromal tumors

Fri Jul 01 00:00:00 UTC 2005

Mitotic cell cycle proteins increase in podocytes despite lack of proliferation.

Kidney international

Petermann AT,Pippin J,Hiromura K,Monkawa T,Durvasula R,Couser WG,Kopp J,Shankland SJ

MA5-13128 was used in western blot to study the increase in mitotic cell cycle protein in podocyes in the absence of increased proliferation

Wed Jan 01 00:00:00 UTC 2003

Citron kinase is a cell cycle-dependent, nuclear protein required for G2/M transition of hepatocytes.

The Journal of biological chemistry

Liu H,Di Cunto F,Imarisio S,Reid LM

MA5-13128 was used in western blot to investigate the role of citron kinase in hepatocyte cell cycle progression

Fri Jan 24 00:00:00 UTC 2003

Kinetics of endomitosis in primary murine megakaryocytes.

Journal of cellular physiology

Carow CE,Fox NE,Kaushansky K

MA5-13128 was used in flow cytometry to investigate the mechanism of endomitosis in primary murine megakaryocytes

Sat Sep 01 00:00:00 UTC 2001

Expression of the ALK protein by anaplastic large-cell lymphomas correlates with high proliferative activity.

International journal of cancer

Leoncini L,Lazzi S,Scano D,Mura A,Onida A,Massarelli G,Tosi P,Barbini P,Cevenini G,Massai MR,Pileri S,Falini B,Giordano A,Kraft R,Laissue JA,Cottier H

MA5-13128 was used in immunohistochemistry to study the correlation between ALK protein expression and the proliferative capacity of anaplastic large-cell lymphomas

Thu Jun 15 00:00:00 UTC 2000

Retinoblastoma-related p107 and pRb2/p130 proteins in malignant lymphomas: distinct mechanisms of cell growth control.

Clinical cancer research : an official journal of the American Association for Cancer Research

Leoncini L,Bellan C,Cossu A,Claudio PP,Lazzi S,Cinti C,Cevenini G,Megha T,Laurini L,Luzi P,Orcioni GF,Piccioli M,Pileri S,Giardino C,Tosi P,Giordano A

MA5-13128 was used in immunohistochemistry to study the expression and role of retinoblastoma protein/p107 and retinoblastoma protein2/p130 in non-Hodgkin's lymphoma

Wed Dec 01 00:00:00 UTC 1999

Expression of p34(cdc2) and cyclins A and B compared to other proliferative features of non-Hodgkin's lymphomas: a multivariate cluster analysis.

International journal of cancer

Leoncini L,Cossu A,Megha T,Bellan C,Lazzi S,Luzi P,Tosi P,Barbini P,Cevenini G,Pileri S,Giordano A,Kraft R,Laissue JA,Cottier H

MA5-13128 was used in immunohistochemistry to perform a multivariate cluster analysis of p34(cdc2), cyclin A and cyclin B expresssion in non-Hodgkin's lymphoma

Fri Oct 08 00:00:00 UTC 1999

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Cyclin B1 Monoclonal Antibody (V152)

Cyclin B1 Antibody (MA5-13128) in ICC/IF

Product Details

MA5-13128

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

We're here to help

Your items have has been added!

Cyclin B1 Monoclonal Antibody (V152)

Cyclin B1 Antibody (MA5-13128) in ICC/IF

Product Details

MA5-13128

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage conditions

Shipping conditions

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

Product Specific Information

Target Information

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