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Invitrogen

Cyclin D1 Monoclonal Antibody (DCS-11)

3 Published Figures

21 References

View all (115) Cyclin D1 antibodies

Datasheet

Protocols

Catalog # MA5-12707

Price (USD)

389.00

This product has been discontinued

500 µL

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Datasheet

Protocols

Published Figures (3)

Application

Cyclin D1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

FIGURE: 1 / 3

Cyclin D1 Antibody (MA5-12707) in WB, IP

Cell reports 2022 - Figure 4. Differential requirements of RB-deficient cells on cyclin E and p130 (A and B) Live cell imaging to track the division of MB436 and MB468 cells treated with palbociclib and/or the knockdown of CDK4 and CDK6 (A). Means and SDs are shown and calculated from triplicate. (B) Co-immunoprecipitation of cyclin D1 showing the failure to assemble complexes with the indicated proteins. (C) Live cell imaging... View More

PMID: 35235778

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Product Details

MA5-12707

Applications

Tested Dilution

Publications

Western Blot (WB)

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 14 publications 14 publications

Functional Assay (Functional)

Assay-dependent

Product Specifications

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

DCS-11

Immunogen

Human recombinant full length cyclin D1 protein

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10986118

Product Specific Information

MA5-12707 targets Cyclin D1/Bcl-1 in FN applications and shows reactivity with Human, mouse, and Rat samples.

The MA5-12707 immunogen is human recombinant full length cyclin D1 protein.

Target Information

Cyclin D1 (PRAD-1, bcl-1) is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. Cyclin D1 is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). In addition, cyclin D1 positively regulates protein phosphorylation, mammary gland epithelial cell proliferation, and fat cell differentiation. In humans, the CCND1 gene encoding cyclin D1 is present on chromosome 11. Cyclin D1 is a cytoplasmic and nuclear protein that is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Without growth factor-mediated stimulation, Cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest. The Cyclin D1 protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The Cyclin D1 protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Cyclin D1 has been successfully employed and is a promising tool for further studies in both cell cycle biology and cancer associated abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CDK/cyclin dependencies define extreme cancer cell-cycle heterogeneity and collateral vulnerabilities.

Cell reports

Knudsen ES,Kumarasamy V,Nambiar R,Pearson JD,Vail P,Rosenheck H,Wang J,Eng K,Bremner R,Schramek D,Rubin SM,Welm AL,Witkiewicz AK

MA5-12707 was used in Immunoprecipitation to define multiple mechanisms for G1/S progression in retinoblastoma-proficient models, which are cyclin-dependent kinase (CDK) 4/6 independent and elicit resistance to FDA-approved inhibitors.

Tue Mar 01 00:00:00 UTC 2022

CDK/cyclin dependencies define extreme cancer cell-cycle heterogeneity and collateral vulnerabilities.

Cell reports

Knudsen ES,Kumarasamy V,Nambiar R,Pearson JD,Vail P,Rosenheck H,Wang J,Eng K,Bremner R,Schramek D,Rubin SM,Welm AL,Witkiewicz AK

Tue Mar 01 00:00:00 UTC 2022

CDK/cyclin dependencies define extreme cancer cell-cycle heterogeneity and collateral vulnerabilities.

Cell reports

Knudsen ES,Kumarasamy V,Nambiar R,Pearson JD,Vail P,Rosenheck H,Wang J,Eng K,Bremner R,Schramek D,Rubin SM,Welm AL,Witkiewicz AK

Tue Mar 01 00:00:00 UTC 2022

p27 allosterically activates cyclin-dependent kinase 4 and antagonizes palbociclib inhibition.

Science (New York, N.Y.)

Guiley KZ,Stevenson JW,Lou K,Barkovich KJ,Kumarasamy V,Wijeratne TU,Bunch KL,Tripathi S,Knudsen ES,Witkiewicz AK,Shokat KM,Rubin SM

MA5-12707 was used in Immunoprecipitation to investigate the mechanism of p27's association with active CDK4.

Fri Dec 13 00:00:00 UTC 2019

CDK4 T172 phosphorylation is central in a CDK7-dependent bidirectional CDK4/CDK2 interplay mediated by p21 phosphorylation at the restriction point.

PLoS genetics

Bisteau X,Paternot S,Colleoni B,Ecker K,Coulonval K,De Groote P,Declercq W,Hengst L,Roger PP

MA5-12707 was used in immunoprecipitation to study the roles CDK7 and p21 in regulating the T172 phosphorylation of CDK4 at the restriction point

Wed May 01 00:00:00 UTC 2013

Aurora B kinase phosphorylates and instigates degradation of p53.

Proceedings of the National Academy of Sciences of the United States of America

Gully CP,Velazquez-Torres G,Shin JH,Fuentes-Mattei E,Wang E,Carlock C,Chen J,Rothenberg D,Adams HP,Choi HH,Guma S,Phan L,Chou PC,Su CH,Zhang F,Chen JS,Yang TY,Yeung SC,Lee MH

MA5-12707 was used in western blot to study the degradation of p53 and the accelerating role of phosphorylation by Aurora B kinase

Tue Jun 12 00:00:00 UTC 2012

Orphan receptor small heterodimer partner suppresses tumorigenesis by modulating cyclin D1 expression and cellular proliferation.

Hepatology (Baltimore, Md.)

Zhang Y,Xu P,Park K,Choi Y,Moore DD,Wang L

MA5-12707 was used in immunoprecipitation to study the mechanism by which the nuclear receptor family member SHP suppresses hepatic carcinogenesis

Tue Jul 01 00:00:00 UTC 2008

Cyclic AMP inhibits the proliferation of thyroid carcinoma cell lines through regulation of CDK4 phosphorylation.

Molecular biology of the cell

Rocha AS,Paternot S,Coulonval K,Dumont JE,Soares P,Roger PP

MA5-12707 was used in immunoprecipitation to study the role of CDK4 phosphorylation in the mechanism by which cAMP inhibits thyroid carcinoma cell proliferation

Sat Nov 01 00:00:00 UTC 2008

Molecular characterization of spontaneous mesenchymal stem cell transformation.

PloS one

Rubio D,Garcia S,Paz MF,De la Cueva T,Lopez-Fernandez LA,Lloyd AC,Garcia-Castro J,Bernad A

MA5-12707 was used in western blot to investigate the molecular mechanisms of spontaneous mesenchymal stem cell transformation

Wed Jan 02 00:00:00 UTC 2008

Cyclin D1 antagonizes BRCA1 repression of estrogen receptor alpha activity.

Cancer research

Wang C,Fan S,Li Z,Fu M,Rao M,Ma Y,Lisanti MP,Albanese C,Katzenellenbogen BS,Kushner PJ,Weber B,Rosen EM,Pestell RG

MA5-12707 was used in western blot to study the opposing effect of cyclin D1 and estrogen receptor on BRCA1 repression

Mon Aug 01 00:00:00 UTC 2005

p21Cip1 and p27Kip1 induce distinct cell cycle effects and differentiation programs in myeloid leukemia cells.

The Journal of biological chemistry

Muñoz-Alonso MJ,Acosta JC,Richard C,Delgado MD,Sedivy J,León J

MA5-12707 was used in immunoprecipitation to study the role of p21Cip1 and p27Kip1 in the cell cycle and differentiation programs of myeloid leukemia cells

Fri May 06 00:00:00 UTC 2005

Cyclin C/cdk3 promotes Rb-dependent G0 exit.

Cell

Ren S,Rollins BJ

MA5-12707 was used in immunoprecipitation to study the role of cyclin C/cdk3 in promoting Rb-dependent G0 exit

Fri Apr 16 00:00:00 UTC 2004

Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts.

The Biochemical journal

Hutter E,Renner K,Pfister G,Stöckl P,Jansen-Dürr P,Gnaiger E

MA5-12707 was used in western blot to study senescence-related changes in respiration and oxidative phosphorylation in primary human fibroblasts

Tue Jun 15 00:00:00 UTC 2004

Differential effects of p21(WAF1/CIP1) deficiency on MMTV-ras and MMTV-myc mammary tumor properties.

Cancer research

Bearss DJ,Lee RJ,Troyer DA,Pestell RG,Windle JJ

MA5-12707 was used in immunoprecipitation to study the role of the CDK inhibitor p21(WAF1/CIP1) in tumor suppression in MMTV-ras and MMTV-myc transgenic mice

Mon Apr 01 00:00:00 UTC 2002

Reconstitution of cyclin D1-associated kinase activity drives terminally differentiated cells into the cell cycle.

Molecular and cellular biology

Latella L,Sacco A,Pajalunga D,Tiainen M,Macera D,D'Angelo M,Felici A,Sacchi A,Crescenzi M

MA5-12707 was used in immunoprecipitation to study the ability of restoring cyclin D1-associated kinase activity to drive terminally differentiated myotubes to enter the cell cycle

Wed Aug 01 00:00:00 UTC 2001

Metabolic stabilization of p27 in senescent fibroblasts correlates with reduced expression of the F-box protein Skp2.

Experimental gerontology

Wagner M,Hampel B,Hütter E,Pfister G,Krek W,Zwerschke W,Jansen-Dürr P

MA5-12707 was used in immunoprecipitation to study the correlation of p27(KIP1) accumulation in senescent fibroblasts with reduced levels of the F-box protein Skp2

Sat Dec 01 00:00:00 UTC 2001

Dominant negative c-jun inhibits activation of the cyclin D1 and cyclin E kinase complexes.

Molecular biology of the cell

Hennigan RF,Stambrook PJ

MA5-12707 was used in immunoprecipitation to investigate the effect of the dominant negative c-jun on the activation of cyclin D1 and cyclin E kinase complexes

Wed Aug 01 00:00:00 UTC 2001

Dominant negative c-jun inhibits activation of the cyclin D1 and cyclin E kinase complexes.

Molecular biology of the cell

Hennigan RF,Stambrook PJ

MA5-12707 was used in immunoprecipitation to investigate the effect of the dominant negative c-jun on the activation of cyclin D1 and cyclin E kinase complexes

Wed Aug 01 00:00:00 UTC 2001

Loss of p27Kip1 from cyclin E/cyclin-dependent kinase (CDK) 2 but not from cyclin D1/CDK4 complexes in cells transformed by polyamine biosynthetic enzymes.

Cancer research

Ravanko K,Järvinen K,Paasinen-Sohns A,Hölttä E

MA5-12707 was used in immunoprecipitation to study the role of p27Kip1 downregulation in tumorigenic transformation induced by polyamine biosynthetic enzyme overexpression

Fri Sep 15 00:00:00 UTC 2000

Progesterone inhibits estrogen-induced cyclin D1 and cdk4 nuclear translocation, cyclin E- and cyclin A-cdk2 kinase activation, and cell proliferation in uterine epithelial cells in mice.

Molecular and cellular biology

Tong W,Pollard JW

MA5-12707 was used in immunohistochemistry to study the molecular mechanisms underlying the effects of female sex hormones on the proliferation of murine uterine epithelial cells

Mon Mar 01 00:00:00 UTC 1999

Inhibition of basal and mitogen-stimulated pancreatic cancer cell growth by cyclin D1 antisense is associated with loss of tumorigenicity and potentiation of cytotoxicity to cisplatinum.

The Journal of clinical investigation

Kornmann M,Arber N,Korc M

MA5-12707 was used in immunoprecipitation to study the effect of cyclin D1 knockdown on the tumorigenicity and cisplantin-sensitivity of PANC-1 human pancreatic cancer cells

Thu Jan 15 00:00:00 UTC 1998

Angiotensin II activation of cyclin D1-dependent kinase activity.

The Journal of biological chemistry

Watanabe G,Lee RJ,Albanese C,Rainey WE,Batlle D,Pestell RG

MA5-12707 was used in immunoprecipitation to investigate the effect of angiotensin II on cyclin D1 expression and cyclin D1-dependent kinase activity in H295R cells

Fri Sep 13 00:00:00 UTC 1996

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Cyclin D1 Monoclonal Antibody (DCS-11)

Cyclin D1 Antibody (MA5-12707) in WB, IP

Product Details

MA5-12707

Western Blot (WB)

Immunohistochemistry (IHC)

Immunoprecipitation (IP)

Functional Assay (Functional)

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

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