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            • Primary Antibodies ›
            • Cytochrome C Antibodies

            Proteintech

            Cytochrome c Polyclonal Antibody

            467 References
            View all (69) Cytochrome C antibodies

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            Datasheet
            Protocols
            Questions & Answers
            Catalog # 10993-1-AP
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            Cite Cytochrome c Polyclonal Antibody

            • Antibody Testing Data (15)
            Application
            Cytochrome c Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Group 53 Created with Sketch.

            FIGURE: 1 / 15

            Cytochrome c Antibody (10993-1-AP) in IHC (P)

            Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10993-1-AP ( Cytochrome c Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
            View Product

            Cytochrome c Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytochrome c Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytochrome c Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytochrome c Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytochrome c Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytochrome c Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytochrome c Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
            Cytochrome c Antibody in Western Blot (WB)
            Cytochrome c Antibody in Western Blot (WB)
            Cytochrome c Antibody in Western Blot (WB)
            Cytochrome c Antibody in Western Blot (WB)
            Cytochrome c Antibody in Western Blot (WB)
            Cytochrome c Antibody in Western Blot (WB)
            Cytochrome c Antibody in Western Blot (WB)
            Cytochrome c Antibody in Western Blot (WB)

            Product Details

            10993-1-AP

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:500-1:1,000
            View 413 publications 413 publications

            Immunohistochemistry (IHC)

            -
            View 22 publications 22 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            1:50-1:500
            -

            Immunohistochemistry (PFA fixed) (IHC (PFA))

            1:200
            -

            Immunocytochemistry (ICC/IF)

            -
            View 20 publications 20 publications

            Flow Cytometry (Flow)

            -
            View 1 publication 1 publication

            Miscellaneous PubMed (Misc)

            -
            View 4 publications 4 publications

            Not applicable (N/A)

            -
            View 7 publications 7 publications
            Product Specifications

            Species Reactivity

            Human, Mouse, Rat

            Published species

            Human, Invertebrate, Mouse, Pig, Plant, Rat, Sheep

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Cytochrome c Fusion Protein Ag1455 (1-105 aa encoded by BC009578)
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.25 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS, pH 7.3, with 50% glycerol

            Contains

            0.02% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Wet ice (domestic); Dry ice (international)

            Product Specific Information

            Immunogen sequence: MGDVEKGKK IFIMKCSQCH TVEKGGKHKT GPNLHGLFGR KTGQAPGYSY TAANKNKGII WGEDTLMEYL ENPKKYIPGT KMIFVGIKKK EERADLIAYL KKATNE (1-105 aa encoded by B C009578)

            Target Information

            Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: CRF; Cytochrome c; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; hc1

            View more View less

            Gene Aliases: CYC; CYCS; CYCSA; HCS; THC4

            View more View less

            UniProt ID: (Human) P99999, (Mouse) P62897, (Rat) P62898

            View more View less

            Entrez Gene ID: (Human) 54205, (Mouse) 13063, (Rat) 25309

            View more View less

            Function(s)

            protein serine/threonine phosphatase activity protein binding heme binding electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity metal ion binding electron carrier activity iron ion binding lipid binding

            Process(es)

            response to reactive oxygen species mitochondrial electron transport, ubiquinol to cytochrome c mitochondrial electron transport, cytochrome c to oxygen protein dephosphorylation mitochondrion organization activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c cellular respiration intrinsic apoptotic signaling pathway apoptotic DNA fragmentation apoptotic process hydrogen peroxide metabolic process ATP synthesis coupled electron transport response to ischemia response to oxidative stress negative regulation of hydrogen peroxide biosynthetic process glial cell apoptotic process positive regulation of cellular respiration
            It has to be done as per old AB suggested Products section.
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