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Cytoglobin (CYGB) Antibody (CF812909) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYGB (RC206642, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYGB (1:500).
Product Details
CF812909
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI5G1
Immunogen
Full length human recombinant protein of human CYGB (NP_599030) produced in HEK293T cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 8% trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress (Trent and Hargrove, 2002).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytoglobin; HGb; Histoglobin; Nitric oxygen dioxygenase; Nitric oxygen dioxygenase CYGB; Nitrite reductase CYGB; NOD; Pseudoperoxidase CYGB; stellate cell activation associated protein; Stellate cell activation-associated protein; Superoxide dismutase CYGB
Gene Aliases: 3110001K20Rik; CYGB; HGB; Staap; STAP
UniProt ID: (Human) Q8WWM9, (Mouse) Q9CX80, (Rat) Q921A4
Entrez Gene ID: (Human) 114757, (Mouse) 114886, (Rat) 170520
transport
response to oxidative stress
negative regulation of fibroblast migration
oxygen transport
fatty acid oxidation
negative regulation of collagen biosynthetic process
oxidation-reduction process
negative regulation of hepatic stellate cell activation
response to hypoxia
regulation of nitric-oxide synthase activity
cellular oxidant detoxification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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