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MUbio
Cytokeratin 10/Keratin K10 Monoclonal Antibody (RKSE60)
Product Details
MUB0319P
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Pig,
Rat,
Zebrafish
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
RKSE60
Immunogen
Cytokeratin 10 / Keratin K10
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
RKSE60 reacts exclusively with Cytokeratin 10 which is present in Keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas.
The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Target Information
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (i.e., skin) and non-keratinizing cells (i.e. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8. 5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 56 kDa cytokeratin; CK-10; CK-17; cytokeratin 10; Cytokeratin-10; cytokeratin-17; epidermal keratin 10; epithelial keratin 10; Epithelial keratin-10; intermediate filament protein; K10; keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris); keratin 10, type I; keratin complex 1, acidic, gene 10; Keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 59 kDa; Keratin-10; keratin-17; krt10 {ECO:0000250|UniProtKB:P13645}; suprabasal cytokeratin 10; type I keratin KA10
Gene Aliases: BCIE; BIE; CK-10; CK10; D130054E02Rik; EHK; K10; K1C1; Ka10; KER10; KPP; Krt-1.10; Krt1-10; KRT10
UniProt ID: (Human) P13645, (Dog) Q6EIZ0, (Mouse) P02535
Entrez Gene ID: (Human) 3858, (Dog) 491006, (Pig) 100519971, (Mouse) 16661, (Rat) 450225
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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