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            • Primary Antibodies ›
            • Cytokeratin 18 Antibodies

            Proteintech

            Cytokeratin 18 Polyclonal Antibody

            97 References
            View all (159) Cytokeratin 18 antibodies

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            Datasheet
            Protocols
            Questions & Answers
            Catalog # 10830-1-AP
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            Price (USD)
            492.00
            150 µL
             
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            Datasheet
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            Cite Cytokeratin 18 Polyclonal Antibody

            • Antibody Testing Data (31)
            Application
            Cytokeratin 18 Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 31

            Cytokeratin 18 Antibody (10830-1-AP) in ICC/IF

            IF result of CK18 antibody (10830-1-AP, 1:50 ) with 1% PLP fixed the APical sustentacular cells of adult mouse olfactory epithelium. (Green: CK18; Blue: DAPI). Basal lamina is marked by an arrow head. By Brian Lin, Tufts University.
            View Product

            Cytokeratin 18 Antibody in Immunocytochemistry (ICC/IF)
            Cytokeratin 18 Antibody in Immunocytochemistry (ICC/IF)
            Cytokeratin 18 Antibody in Immunocytochemistry (ICC/IF)
            Cytokeratin 18 Antibody in Immunocytochemistry (ICC/IF)
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Cytokeratin 18 Antibody in Western Blot (WB)
            Cytokeratin 18 Antibody in Western Blot (WB)
            Cytokeratin 18 Antibody in Western Blot (WB)
            Cytokeratin 18 Antibody in Flow Cytometry (Flow)
            Cytokeratin 18 Antibody in Immunoprecipitation (IP)

            Product Details

            10830-1-AP

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:1,000-1:4,000
            View 30 publications 30 publications

            Immunohistochemistry (IHC)

            -
            View 21 publications 21 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            1:200-1:1,000
            -

            Immunocytochemistry (ICC/IF)

            1:50-1:500
            View 38 publications 38 publications

            Flow Cytometry (Flow)

            0.2 µg/1x10^6 cells
            View 2 publications 2 publications

            Immunoprecipitation (IP)

            0.5-4.0 µg
            -

            Miscellaneous PubMed (Misc)

            -
            View 1 publication 1 publication

            Not applicable (N/A)

            -
            View 5 publications 5 publications
            Product Specifications

            Species Reactivity

            Human, Mouse

            Published species

            Human, Mouse, Rat

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Cytokeratin 18 Fusion Protein Ag1260 (127-430 aa encoded by BC000180)
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.19 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS, pH 7.3, with 50% glycerol

            Contains

            0.02% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Wet ice (domestic); Dry ice (international)

            Product Specific Information

            Immunogen sequence: SHYFKIIED LRAQIFANTV DNARIVLQID NARLAADDFR VKYETELAMR QSVENDIHGL RKVIDDTNIT RLQLETEIEA LKEELLFMKK NHEEEVKGLQ AQIASSGLTV EVDAPKSQDL AKIMADIRAQ YDELARKNRE ELDKYWSQQI EESTTVVTTQ SAEVGAAETT LTELRRTVQS LEIDLDSMRN LKASLENSLR EVEARYALQM EQLNGILLHL ESELAQTRAE GQRQAQEYEA LLNIKVKLEA EIATYRRLLE DGEDFNLGDA LDSSNSMQTI QKTTTRRIVD GKVVSETNDT KVLRH (127-430 aa encoded by BC000180)

            Target Information

            Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: Cell proliferation-inducing gene 46 protein; CK 18; CK-18; cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; Cytokeratin18; Endo B; K 18; K18; keratin 18, type I; keratin complex 1, acidic, gene 18; Keratin D; Keratin, type I cytoskeletal 18; Keratin-18; Keratin18; KRT 18; type I cytoskeletal 18

            View more View less

            Gene Aliases: CK-18; CK18; CYK18; K18; Kerd; Krt1-18; KRT18; PIG46

            View more View less

            UniProt ID: (Human) P05783, (Mouse) P05784

            View more View less

            Entrez Gene ID: (Human) 3875, (Mouse) 16668

            View more View less

            Function(s)

            structural molecule activity protein binding poly(A) RNA binding scaffold protein binding cadherin binding involved in cell-cell adhesion

            Process(es)

            cell cycle anatomical structure morphogenesis viral process tumor necrosis factor-mediated signaling pathway Golgi to plasma membrane CFTR protein transport negative regulation of apoptotic process intermediate filament cytoskeleton organization extrinsic apoptotic signaling pathway hepatocyte apoptotic process cell-cell adhesion
            It has to be done as per old AB suggested Products section.
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