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Application
FIGURE: 1 / 1
D2HGDH Antibody (PA5-95720) in WB
Western blot analysis of D2HGDH in extracts of various cell lines. Samples were incubated with D2HGDH Polyclonal antibody (Product # PA5-95720) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-95720
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 322-521 of human D2HGDH (NP_6899964)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.91 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807522
Product Specific Information
Immunogen sequence: GRHLHLASPV QESPFYVLIE TSGSNAGHDA EKLGHFLEHA LGSGLVTDGT MATDQRKVKM LWALRERITE ALSRDGYVYK YDLSLPVERL YDIVTDLRAR LGPHAKHVVG YGHLGDGNLH LNVTAEAFSP SLLAALEPHV YEWTAGQQGS VSAEHGVGFR KRDVLGYSKP PGALQLMQQL KALLDPKGIL NPYKTLPSQA; Positive Samples: Mouse liver, Rat heart
Target Information
This gene encodes D-2hydroxyglutarate dehydrogenase, a mitochondrial enzyme belonging to the FAD-binding oxidoreductase/transferase type 4 family. This enzyme, which is most active in liver and kidney but also active in heart and brain, converts D-2-hydroxyglutarate to 2-ketoglutarate. Mutations in this gene are present in D-2-hydroxyglutaric aciduria, a rare recessive neurometabolic disorder causing developmental delay, epilepsy, hypotonia, and dysmorphic features.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: D-2-hydroxyglutarate dehydrogenase, mitochondrial; D2hgdh {ECO:0000250|UniProtKB:Q8N465}; FLJ42195; MGC25181
Gene Aliases: AA408776; AA408778; AI325464; D2hgdh; RGD1307976
UniProt ID: (Mouse) Q8CIM3, (Rat) P84850
Entrez Gene ID: (Mouse) 98314, (Rat) 301624
catalytic activity
UDP-N-acetylmuramate dehydrogenase activity
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
limonene-1,2-diol dehydrogenase activity
flavin adenine dinucleotide binding
(R)-2-hydroxyglutarate dehydrogenase activity
dehydrogenase
oxidoreductase
metabolite interconversion enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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