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DAP3 Antibody (PA5-96541) in WB
Western blot analysis of DAP3 in extracts of various cell lines. Samples were incubated with DAP3 Polyclonal antibody (Product # PA5-96541) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-96541
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 159-398 of human DAP3 (NP_3875061)
Conjugate
Form
Liquid
Concentration
3.4 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808343
Product Specific Information
Immunogen sequence: HLWVKNCRDL LQSSYNKQRF DQPLEASTWL KNFKTTNERF LNQIKVQEKY VWNKRESTEK GSPLGEVVEQ GITRVRNATD AVGIVLKELK RQSSLGMFHL LVAVDGINAL WGRTTLKRED KSPIAPEELA LVHNLRKMMK NDWHGGAIVS ALSQTGSLFK PRKAYLPQEL LGKEGFDALD PFIPILVSNY NPKEFESCIQ YYLENNWLQH EKAPTEEGKK ELLFLSNANP SLLERHCAYL; Positive Samples: MCF7, Jurkat, Mouse kidney, Mouse liver; Cellular Location: Mitochondrion
Target Information
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 28S ribosomal protein S29, mitochondrial; DAP-3; Death-associated protein 3; DKFZp686G12159; Ionizing radiation resistance conferring protein; MGC126058; MGC126059; mitochondrial 28S ribosomal protein S29; MRP-S29; S29mt; Small ribosomal subunit protein mS29
Gene Aliases: 4921514D13Rik; bMRP-10; DAP-3; DAP3; MRP-S29; MRPS29; S29mt
UniProt ID: (Human) P51398, (Mouse) Q9ER88
Entrez Gene ID: (Human) 7818, (Rat) 295238, (Mouse) 65111
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