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Invitrogen
DAPK1 Monoclonal Antibody (12B1)
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Application
FIGURE: 1 / 6
DAPK1 Antibody (MA5-36128) in ICC/IF
Immunocytochemical analysis of DAP Kinase 1 in MCF-7 cells using a DAP Kinase 1 monoclonal antibody (Product #MA5-36128). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI.
Product Details
MA5-36128
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
12B1
Immunogen
Recombinant protein within Human DAPK1 aa 447-683
Conjugate
Unconjugated
Form
Liquid
Concentration
2 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.2% BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2890436
Target Information
DAPK1 is a large, calmodulin-dependent kinase involved in gamma-interferon induced programmed cell death and is a potential tumor suppressor. DAPK1 is involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and authophagy. It regulates both type I apoptotic and type II autophagic cell death signal, depending on the cellular setting. Type I apoptotic cell death is caspase-dependent, type II authophagic cell death is caspase-independent. DAPK1 phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. It also phosphorylates TPM1, STX1A, PRKD1, BECN1, TSC2, RPS6, MYL9, and DAPK3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DAP kinase 1; Death-associated protein kinase 1; DKFZp781I035; OTTHUMP00000216193
Gene Aliases: D13Ucla1; DAP-Kinase; DAPK; DAPK1
UniProt ID: (Human) P53355, (Mouse) Q80YE7
Entrez Gene ID: (Human) 1612, (Rat) 306722, (Mouse) 69635
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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