Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
DC-SIGN Monoclonal Antibody (UW60.1), Alexa Fluor™ 647
FIGURE: 1 / 2
DC-SIGN Antibody (MA5-44144) in Flow
Separation of human CD209 positive cells (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human human GM-CSF and IL-4 stimulated peripheral blood mononuclear cells stained using anti-human CD209 (UW60.1) Alexa Fluor® 647 Monoclonal antibody (Product # MA5-44144) using a dilution of 4 µL reagent/100 µL of peripheral whole blood.
Product Details
MA5-44144
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
UW60.1
Immunogen
CD209-His-tagged fusion protein
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
PBS with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913076
Product Specific Information
This antibody recognizes an extracellular epitope of human CD209 (DC-SIGN), a 44 kDa transmembrane receptor, expressed on the surface of dendritic cells and macrophages.
Target Information
This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: C-type lectin domain family 4 member L; C-type lectin domain family 4, member L; CD209; CD209 antigen; DC-SIGN; Dendritic cell-specific ICAM-3-grabbing non-integrin 1; dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin; dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin; HIV gpl20-binding protein; MGC129965; MGC130443
Gene Aliases: CD209; CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1
UniProt ID: (Human) Q9NNX6
Entrez Gene ID: (Human) 30835
stimulatory C-type lectin receptor signaling pathway
adaptive immune response
endocytosis
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell-cell recognition
modulation by virus of host morphology or physiology
virion attachment to host cell
viral genome replication
antigen processing and presentation
intracellular signal transduction
regulation of T cell proliferation
innate immune response
viral entry into host cell
peptide antigen transport
intracellular transport of virus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support