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DDX1 Antibody (A300-520A-M) in WB
Detection of human DDX1 by western blot. Samples: Whole cell lysate (20, 40 and 80 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-DDX1 antibody (Cat. No. A300-520A-M) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A300-520A-M
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residues 500 and 550 of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.20 mg/mL
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, Bovine and Orangutan
Target Information
DDX1 (ATP-dependent RNA helicase DDX1) acts as an ATP-dependent RNA helicase. It is able to unwind both RNA-RNA and RNA-DNA duplexes. DDX1 possesses 5' single-stranded RNA overhand nuclease activity and ATPase activity on various RNA, but not DNA polynucleotides. DDX1, together with RELA, acts as a coactivator to enhance NF-kappa-b-mediated transcriptional activation. It also acts as a positive transcriptional regulator of cyclin CCND2 expression. It binds to the cyclin CCND2 promoter region. DDX1 is a component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double stranded RNA and plays a role in activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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