Proteintech

DDX20 Polyclonal Antibody

DDX20 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

DDX20 Antibody (11324-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using DDX20 antibody 11324-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

DDX20 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DDX20 Antibody in Immunoprecipitation (IP)

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Product Details

11324-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DDX20 Fusion Protein Ag1863 (520-824 aa encoded by BC011556)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.53 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SHSECGIIE KATSPKELGC DRQSEEQMKN SVQTPVENST NSQHQVKEAL PVSLPQIPCL SSFKIHQPYT LTFAELVEDY EHYIKEGLEK PVEIIRHYTG PGDQTVNPQN GFVRNKVTEQ RVPVLASSSQ SGDSESDSDS YSSRTSSQSK GNKSYLEGSS DNQLKDSEST PVDDRISLEQ PPNGSDTPNP EKYQESPGIQ MKTRLKEGAS QRAKQSRRNL PRRSSFRLQT EAQEDDWYDC HREIRLSFSD TYQDYEEYWR AYYRAWQEYY AAASHSYYWN AQRHPSWMAA YHMNTIYLQE MMHSNQ (520-824 aa encoded by BC011556)

Target Information

Gemin 3 is a member of the DEAD box protein family which are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and are putative RNA helicases. DEAD box proteins such as Gemin 3 are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of DEAD box family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Gemin 3 has an ATPase activity and a component of the survival of motor neurons (SMN) complex. The Gemin 3 protein may play a catalytic role in the function of the SMN complex on RNPs. Diseases associated with Gemin 3 protein dysfunction include spinal muscular dystrophy and muscular atrophy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An artificial neural network model to diagnose non-obstructive azoospermia based on RNA-binding protein-related genes.

Aging

Peng F,Muhuitijiang B,Zhou J,Liang H,Zhang Y,Zhou R

Mon Apr 24 00:00:00 EDT 2023

Analysis of the expression, function, prognosis and co-expression genes of DDX20 in gastric cancer.

Computational and structural biotechnology journal

Wang Q,Ye Y,Lin R,Weng S,Cai F,Zou M,Niu H,Ge L,Lin Y

Sat Oct 03 00:00:00 EDT 2020

Integrating transcriptome-wide study and mRNA expression profiles yields novel insights into the biological mechanism of chondropathies.

Arthritis research & therapy

Li P,Ning Y,Guo X,Wen Y,Cheng B,Ma M,Zhang L,Cheng S,Wang S,Zhang F

Tue Aug 27 00:00:00 EDT 2019

Gemin5 plays a role in unassembled-U1 snRNA disposal in SMN-deficient cells.

FEBS letters

Jiang D,Zou X,Zhang C,Chen J,Li Z,Wang Y,Deng Z,Wang L,Chen S

Sun Apr 01 00:00:00 EDT 2018

High expression of DDX20 enhances the proliferation and metastatic potential of prostate cancer cells through the NF-κB pathway.

International journal of molecular medicine

Chen W,Zhou P,Li X

Wed Jun 01 00:00:00 EDT 2016

High expression of DDX20 enhances the proliferation and metastatic potential of prostate cancer cells through the NF-κB pathway.

International journal of molecular medicine

Chen W,Zhou P,Li X

Wed Jun 01 00:00:00 EDT 2016

High expression of DDX20 enhances the proliferation and metastatic potential of prostate cancer cells through the NF-κB pathway.

International journal of molecular medicine

Chen W,Zhou P,Li X

Wed Jun 01 00:00:00 EDT 2016

High expression of DDX20 enhances the proliferation and metastatic potential of prostate cancer cells through the NF-κB pathway.

International journal of molecular medicine

Chen W,Zhou P,Li X

Wed Jun 01 00:00:00 EDT 2016

FOXL2 down-regulates vitellogenin expression at mature stage in Eriocheir sinensis.

Bioscience reports

Li Q,Xie J,He L,Wang Y,Yang H,Duan Z,Wang Q

Thu Oct 01 00:00:00 EDT 2015

DEAD-box helicase DP103 defines metastatic potential of human breast cancers.

The Journal of clinical investigation

Shin EM,Hay HS,Lee MH,Goh JN,Tan TZ,Sen YP,Lim SW,Yousef EM,Ong HT,Thike AA,Kong X,Wu Z,Mendoz E,Sun W,Salto-Tellez M,Lim CT,Lobie PE,Lim YP,Yap CT,Zeng Q,Sethi G,Lee MB,Tan P,Goh BC,Miller LD,Thiery JP,Zhu T,Gaboury L,Tan PH,Hui KM,Yip GW,Miyamoto S,Kumar AP,Tergaonkar V

Mon Sep 01 00:00:00 EDT 2014

DEAD-box helicase DP103 defines metastatic potential of human breast cancers.

The Journal of clinical investigation

Mon Sep 01 00:00:00 EDT 2014

DEAD-box helicase DP103 defines metastatic potential of human breast cancers.

The Journal of clinical investigation

Mon Sep 01 00:00:00 EDT 2014

DEAD-box helicase DP103 defines metastatic potential of human breast cancers.

The Journal of clinical investigation

Mon Sep 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Component of gems 3; DDX 20; DEAD (Asp-Glu-Ala-Asp) box polypeptide 20; DEAD box protein 20; DEAD box protein DP 103; DEAD-box protein DP103; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD; DEAD/H BOX 20; DKFZP434H052; DP 103; Gemin-3; Probable ATP-dependent RNA helicase DDX20; Regulator of steroidogenic factor 1; ROSF-1; SMN-interacting protein

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Gene Aliases: DDX20; DP103; GEMIN3

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UniProt ID: (Human) Q9UHI6, (Mouse) Q9JJY4

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Entrez Gene ID: (Human) 11218, (Mouse) 53975

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Function(s)

DNA binding ATP-dependent RNA helicase activity protein binding ATP binding protein domain specific binding protein binding, bridging histone deacetylase binding repressing transcription factor binding nucleotide binding nucleic acid binding helicase activity hydrolase activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter spliceosomal tri-snRNP complex assembly spliceosomal snRNP assembly RNA processing negative regulation of cell proliferation RNA secondary structure unwinding positive regulation of apoptotic process oogenesis regulation of steroid biosynthetic process nuclear import mRNA processing RNA splicing negative regulation of transcription, DNA-templated

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DDX20 Polyclonal Antibody

DDX20 Antibody (11324-1-AP) in ICC/IF

Product Details

11324-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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