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Bethyl Laboratories
DDX28 Polyclonal Antibody
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Application
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DDX28 Antibody (A300-762A) in WB
Detection of human DDX28 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DDX28 antibody (Product # A300-762A) (lot A300-762A-1) used for WB at 0.4 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate (B). DDX28 was also immunoprecipitated using rabbit anti-DDX28 antibody BL3236 at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 minutes (... View More
Product Details
A300-762A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 500 and the C-terminus (residue 540) of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 28.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
WDR11 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is located in the chromosome 10q25-26 region, which is frequently deleted in gliomas and tumors of other tissues, and is disrupted by the t(10;19) translocation rearrangement in glioblastoma cells. The gene location suggests that it is a candidate gene for the tumor suppressor locus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DEAD (Asp-Glu-Ala-Asp) box polypeptide 28; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 28; Mitochondrial DEAD box protein 28; mitochondrial DEAD-box polypeptide 28; Probable ATP-dependent RNA helicase DDX28
Gene Aliases: DDX28; MDDX28
UniProt ID: (Human) Q9NUL7
Entrez Gene ID: (Human) 55794
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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