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Invitrogen
DEPDC6 Recombinant Rabbit Monoclonal Antibody (PH00-03)
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Application
FIGURE: 1 / 6
DEPDC6 Antibody (MA5-45977) in ICC/IF
Immunofluorescence analysis of DEPDC6 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DEPDC6 Recombinant Rabbit Monoclonal Antibody (PH00-03) (Product # MA5-45977) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Flu... View More
Product Details
MA5-45977
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PH00-03
Immunogen
Recombinant protein within human DPTOR aa 1-300.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2937398
Product Specific Information
Predicted band size: 46 kDa
Positive Control: MCF-7 cell lysate, Daudi cell lysate, PC-3M cell lysate, N2A cell lysate, L6 cell lysate, mouse brain tissue lysate, human small intestine tissue.
Sequence Similarities: 96.35%Mouse; 97.73% Rat.
Tissue Specificity: Expression is negatively regulated by both mTORC1 and mTORC2 (at protein level).
Target Information
Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DEP domain containing 6; DEP domain containing mTOR interacting protein; DEP domain containing MTOR-interacting protein; DEP domain-containing mTOR-interacting protein; DEP domain-containing protein 6; hDEPTOR
Gene Aliases: DEP.6; DEPDC6; DEPTOR; Kiaa4200; RGD1561030
UniProt ID: (Human) Q8TB45, (Mouse) Q570Y9
Entrez Gene ID: (Human) 64798, (Mouse) 97998, (Rat) 314979
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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