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OriGene
DGKB Monoclonal Antibody (OTI1F9), TrueMAB™
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Application
FIGURE: 1 / 2
DGKB Antibody (CF501381) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DGKB (RC211233, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-DGKB. Positive lysates LY407898 (100 µg) and LC407898 (20 µg) can be purchased separately from OriGene.
Product Details
CF501381
Product Specifications
Species Reactivity
Dog,
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1F9
Immunogen
Full length human recombinant protein of human DGKB produced in HEK293T cell.
Conjugate
Unconjugated
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Diacylglycerol (DAG) is an allosteric activator of protein kinase C. DAG also participates in regulating RAS and RHO family proteins by activating the guanine nucleotide exchange factors VAV and RASGRP1. DAG is also involved in the synthesis of phospholipids and triacylglycerols. Tight regulation of DAG levels is achieved via DAG kinases (DGKs), which remove DAG by phosphorylate it to phosphatidic acid. Several mammalian isozymes of DAGK have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 90 kDa diacylglycerol kinase; DAG kinase beta; DGK-beta; Diacylglycerol kinase beta; diacylglycerol kinase, beta 90kDa; Diglyceride kinase beta
Gene Aliases: DAGK2; DGK; DGK-BETA; DGKB; KIAA0718
UniProt ID: (Human) Q9Y6T7
Entrez Gene ID: (Human) 1607, (Dog) 482328
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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