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Bethyl Laboratories
DHCR7 Polyclonal Antibody
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Application
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DHCR7 Antibody (A305-321A) in IP
Detection of human DHCR7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DHCR7 antibody (Product # A305-321A) (lot 1) used for IP at 6 µg per reaction. DHCR7 was also immunoprecipitated by rabbit anti-DHCR7 antibody BL21078. For blotting immunoprecipitated DHCR7, A305-321A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A305-321A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1 to 50 of human 7-dehydrocholesterol reductase.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 7-dehydrocholesterol reductase; 7-DHC reductase; delta-7-dehydrocholesterol reductase; Delta7-sterol reductase; putative sterol reductase SR-2; Sterol Delta(7)-reductase; sterol delta-7-reductase; Sterol reductase SR-2
Gene Aliases: D7SR; DHCR7; SLOS
UniProt ID: (Human) Q9UBM7
Entrez Gene ID: (Human) 1717
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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