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DIAPH3 Polyclonal Antibody

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Antibody Testing Data (11)

DIAPH3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

DIAPH3 Antibody (14342-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 14342-1-AP (DIAPH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

DIAPH3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DIAPH3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14342-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 6 publications 6 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DIAPH3 Fusion Protein Ag5656 (500-850 aa encoded by BC048963)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: DQEQLNSLS QFKSEYSNLC EPEQFVVVMS NVKRLRPRLS AILFKLQFEE QVNNIKPDIM AVSTACEEIK KSKSFSKLLE LVLLMGNYMN AGSRNAQTFG FNLSSLCKLK DTKSADQKTT LLHFLVEICE EKYPDILNFV DDLEPLDKAS KVSVETLEKN LRQMGRQLQQ LEKELETFPP PEDLHDKFVT KMSRFVISAK EQYETLSKLH ENMEKLYQSI IGYYAIDVKK VSVEDFLTDL NNFRTTFMQA IKENIKKREA EEKEKRVRIA KELAERERLE RQQKKKRLLE MKTEGDETGV MDNLLEALQS GAAFRDRRKR TPMPKDVRQS LSPMSQRPVL KVCNHGNKPY L (500-850 aa encoded by BC048963)

Target Information

This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The pan-cancer analysis identified DIAPH3 as a diagnostic biomarker of clinical cancer.

Aging

Chen X,Xie L,Qiao K,Zhu X,Ren J,Tan Y

Thu Jan 05 00:00:00 EST 2023

The pan-cancer analysis identified DIAPH3 as a diagnostic biomarker of clinical cancer.

Aging

Chen X,Xie L,Qiao K,Zhu X,Ren J,Tan Y

Thu Jan 05 00:00:00 EST 2023

Formin-mediated nuclear actin at androgen receptors promotes transcription.

Nature

Knerr J,Werner R,Schwan C,Wang H,Gebhardt P,Grötsch H,Caliebe A,Spielmann M,Holterhus PM,Grosse R,Hornig NC

Mon May 01 00:00:00 EDT 2023

TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.

The EMBO journal

Huang Q,Wu D,Zhao J,Yan Z,Chen L,Guo S,Wang D,Yuan C,Wang Y,Liu X,Xing J

Wed Jun 01 00:00:00 EDT 2022

TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.

The EMBO journal

Huang Q,Wu D,Zhao J,Yan Z,Chen L,Guo S,Wang D,Yuan C,Wang Y,Liu X,Xing J

Wed Jun 01 00:00:00 EDT 2022

TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.

The EMBO journal

Huang Q,Wu D,Zhao J,Yan Z,Chen L,Guo S,Wang D,Yuan C,Wang Y,Liu X,Xing J

Wed Jun 01 00:00:00 EDT 2022

The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells.

Cells

Becker KN,Pettee KM,Sugrue A,Reinard KA,Schroeder JL,Eisenmann KM

Thu May 05 00:00:00 EDT 2022

DIAPH3 is a prognostic biomarker and inhibit colorectal cancer progression through maintaining EGFR degradation.

Cancer medicine

Huang R,Wu C,Wen J,Yu J,Zhu H,Yu J,Zou Z

Thu Dec 01 00:00:00 EST 2022

DIAPH3 is a prognostic biomarker and inhibit colorectal cancer progression through maintaining EGFR degradation.

Cancer medicine

Huang R,Wu C,Wen J,Yu J,Zhu H,Yu J,Zou Z

Thu Dec 01 00:00:00 EST 2022

Correction: Generation of stress fibers through myosin-driven reorganization of the actin cortex.

eLife

Lehtimäki JI,Rajakylä EK,Tojkander S,Lappalainen P

Wed Apr 20 00:00:00 EDT 2022

Membrane skeleton modulates erythroid proteome remodeling and organelle clearance.

Blood

Liu Y,Mei Y,Han X,Korobova FV,Prado MA,Yang J,Peng Z,Paulo JA,Gygi SP,Finley D,Ji P

Thu Jan 21 00:00:00 EST 2021

Nonredundant roles of DIAPHs in primary ciliogenesis.

The Journal of biological chemistry

Palander O,Lam A,Collins RF,Moraes TJ,Trimble WS

Fri Aug 20 00:00:00 EDT 2021

Overexpression of the microtubule-binding protein CLIP-170 induces a +TIP network superstructure consistent with a biomolecular condensate.

PloS one

Wu YO,Bryant AT,Nelson NT,Madey AG,Fernandes GF,Goodson HV

Thu Jan 13 00:00:00 EST 2022

Generation of stress fibers through myosin-driven reorganization of the actin cortex.

eLife

Lehtimäki JI,Rajakylä EK,Tojkander S,Lappalainen P

Thu Jan 28 00:00:00 EST 2021

Recessive NOS1AP variants impair actin remodeling and cause glomerulopathy in humans and mice.

Science advances

Majmundar AJ,Buerger F,Forbes TA,Klämbt V,Schneider R,Deutsch K,Kitzler TM,Howden SE,Scurr M,Tan KS,Krzeminski M,Widmeier E,Braun DA,Lai E,Ullah I,Amar A,Kolb A,Eddy K,Chen CH,Salmanullah D,Dai R,Nakayama M,Ottlewski I,Kolvenbach CM,Onuchic-Whitford AC,Mao Y,Mann N,Nabhan MM,Rosen S,Forman-Kay JD,Soliman NA,Heilos A,Kain R,Aufricht C,Mane S,Lifton RP,Shril S,Little MH,Hildebrandt F

Fri Jan 01 00:00:00 EST 2021

Targeting the mDia Formin-Assembled Cytoskeleton Is an Effective Anti-Invasion Strategy in Adult High-Grade Glioma Patient-Derived Neurospheres.

Cancers

Pettee KM,Becker KN,Alberts AS,Reinard KA,Schroeder JL,Eisenmann KM

Wed Mar 20 00:00:00 EDT 2019

Targeting the mDia Formin-Assembled Cytoskeleton Is an Effective Anti-Invasion Strategy in Adult High-Grade Glioma Patient-Derived Neurospheres.

Cancers

Pettee KM,Becker KN,Alberts AS,Reinard KA,Schroeder JL,Eisenmann KM

Wed Mar 20 00:00:00 EDT 2019

Formin-dependent TGF-β signaling for epithelial to mesenchymal transition.

Molecular biology of the cell

Rana MK,Aloisio FM,Choi C,Barber DL

Fri Jun 15 00:00:00 EDT 2018

Formin-dependent TGF-β signaling for epithelial to mesenchymal transition.

Molecular biology of the cell

Rana MK,Aloisio FM,Choi C,Barber DL

Fri Jun 15 00:00:00 EDT 2018

Carcinoma associated fibroblasts (CAFs) promote breast cancer motility by suppressing mammalian Diaphanous-related formin-2 (mDia2).

PloS one

Dvorak KM,Pettee KM,Rubinic-Minotti K,Su R,Nestor-Kalinoski A,Eisenmann KM

Tue Jul 24 00:00:00 EDT 2018

Carcinoma associated fibroblasts (CAFs) promote breast cancer motility by suppressing mammalian Diaphanous-related formin-2 (mDia2).

PloS one

Dvorak KM,Pettee KM,Rubinic-Minotti K,Su R,Nestor-Kalinoski A,Eisenmann KM

Tue Jul 24 00:00:00 EDT 2018

Actomyosin stress fiber subtypes have unique viscoelastic properties and roles in tension generation.

Molecular biology of the cell

Lee S,Kassianidou E,Kumar S

Wed Aug 08 00:00:00 EDT 2018

APLP2, RRM2, and PRC1: New Putative Markers for the Differential Diagnosis of Thyroid Follicular Lesions.

Thyroid : official journal of the American Thyroid Association

Castelblanco E,Zafon C,Maravall J,Gallel P,Martinez M,Capel I,Bella MR,Halperin I,Temprana J,Iglesias C,Puig-Domingo M,Robledo M,Matias-Guiu X,Mauricio D

Sun Jan 01 00:00:00 EST 2017

APLP2, RRM2, and PRC1: New Putative Markers for the Differential Diagnosis of Thyroid Follicular Lesions.

Thyroid : official journal of the American Thyroid Association

Castelblanco E,Zafon C,Maravall J,Gallel P,Martinez M,Capel I,Bella MR,Halperin I,Temprana J,Iglesias C,Puig-Domingo M,Robledo M,Matias-Guiu X,Mauricio D

Sun Jan 01 00:00:00 EST 2017

mDia2 and CXCL12/CXCR4 chemokine signaling intersect to drive tumor cell amoeboid morphological transitions.

Biochemical and biophysical research communications

Wyse MM,Goicoechea S,Garcia-Mata R,Nestor-Kalinoski AL,Eisenmann KM

Sat Mar 04 00:00:00 EST 2017

mDia2 and CXCL12/CXCR4 chemokine signaling intersect to drive tumor cell amoeboid morphological transitions.

Biochemical and biophysical research communications

Wyse MM,Goicoechea S,Garcia-Mata R,Nestor-Kalinoski AL,Eisenmann KM

Sat Mar 04 00:00:00 EST 2017

mDia2 and CXCL12/CXCR4 chemokine signaling intersect to drive tumor cell amoeboid morphological transitions.

Biochemical and biophysical research communications

Wyse MM,Goicoechea S,Garcia-Mata R,Nestor-Kalinoski AL,Eisenmann KM

Sat Mar 04 00:00:00 EST 2017

mDia2 and CXCL12/CXCR4 chemokine signaling intersect to drive tumor cell amoeboid morphological transitions.

Biochemical and biophysical research communications

Wyse MM,Goicoechea S,Garcia-Mata R,Nestor-Kalinoski AL,Eisenmann KM

Sat Mar 04 00:00:00 EST 2017

An mDia2/ROCK signaling axis regulates invasive egress from epithelial ovarian cancer spheroids.

PloS one

Pettee KM,Dvorak KM,Nestor-Kalinoski AL,Eisenmann KM

Tue Oct 28 00:00:00 EDT 2014

An mDia2/ROCK signaling axis regulates invasive egress from epithelial ovarian cancer spheroids.

PloS one

Pettee KM,Dvorak KM,Nestor-Kalinoski AL,Eisenmann KM

Tue Oct 28 00:00:00 EDT 2014

Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation.

Cell research

Stastna J,Pan X,Wang H,Kollmannsperger A,Kutscheidt S,Lohmann V,Grosse R,Fackler OT

Sun Apr 01 00:00:00 EDT 2012

Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation.

Cell research

Stastna J,Pan X,Wang H,Kollmannsperger A,Kutscheidt S,Lohmann V,Grosse R,Fackler OT

Sun Apr 01 00:00:00 EDT 2012

The formin FMNL3 is a cytoskeletal regulator of angiogenesis.

Journal of cell science

Hetheridge C,Scott AN,Swain RK,Copeland JW,Higgs HN,Bicknell R,Mellor H

Thu Mar 15 00:00:00 EDT 2012

The formin FMNL3 is a cytoskeletal regulator of angiogenesis.

Journal of cell science

Hetheridge C,Scott AN,Swain RK,Copeland JW,Higgs HN,Bicknell R,Mellor H

Thu Mar 15 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: diaphanous homolog 3; diaphanous-related formin 3; Diaphanous-related formin-3; DKFZp434C0931; DKFZp686A13178; DRF3; FLJ34705; MDia2; Protein diaphanous homolog 3

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Gene Aliases: AN; AUNA1; DIA2; DIAP3; DIAPH3; DRF3; mDia2; NSDAN

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UniProt ID: (Human) Q9NSV4

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Entrez Gene ID: (Human) 81624

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Function(s)

actin binding Rho GTPase binding cadherin binding involved in cell-cell adhesion

Process(es)

spermatogenesis actin cytoskeleton organization cell-cell adhesion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DIAPH3 Polyclonal Antibody

DIAPH3 Antibody (14342-1-AP) in ICC/IF

Product Details

14342-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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