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Invitrogen
DMPO Monoclonal Antibody (N1664A)
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Application
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DMPO Antibody (MA5-27550) in ICC/IF
Immunofluorescent analysis of DMPO in mouse macrophage cell line (raw 264.7). Sample was incubated with DMPO monoclonal antibody (Product # MA5-27550) using a dilution of 1:100, and followed by Alexa Fluor 488 Goat Anti-Mouse (green) and DAPI (blue) secondary antibody at a dilution of 1:1000 (1 hour at RT).
Product Details
MA5-27550
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N1664A
Immunogen
5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735169
Product Specific Information
A 1:1,000 dilution of MA5-27550 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.
Target Information
The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems. Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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