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Invitrogen

DNA-PK Polyclonal Antibody

1 Published Figure

1 Reference

View all (51) DNA-PK antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (1)

Application

DNA-PK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 3

DNA-PK Antibody (PA5-86134) in IHC (P)

Immunohistochemical analysis of DNA-PK in paraffin-embedded human breast carcinoma tissue. Sample was incubated with polyclonal DNA-PK antibody (Product # PA5-86134) using a 1:100 dilution.

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Product Details

PA5-86134

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide, corresponding to amino acids 4060-4110 of Human DNA-PKCS.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2802929

Product Specific Information

Purity is > 95% by SDS-PAGE.

Target Information

DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. DNA-PK is an atypical kinase, closely related to the PI3 kinases, and is often potently inhibited by small molecules designed to target PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for DNA-PK.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Divergent FUS phosphorylation in primate and mouse cells following double-strand DNA damage.

Neurobiology of disease

Johnson MA,Deng Q,Taylor G,McEachin ZT,Chan AWS,Root J,Bassell GJ,Kukar T

Published figure using DNA-PK polyclonal antibody (Product # PA5-86134) in Western Blot

Tue Dec 01 00:00:00 UTC 2020

Divergent FUS phosphorylation in primate and mouse cells following double-strand DNA damage.

Neurobiology of disease

Johnson MA,Deng Q,Taylor G,McEachin ZT,Chan AWS,Root J,Bassell GJ,Kukar T

Published figure using DNA-PK polyclonal antibody (Product # PA5-86134) in Western Blot

Tue Dec 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNPK1; doxorubicin nephropathy; hyper-radiosensitivity of murine scid mutation, complementing 1; p460; severe combined immunodeficiency

Gene Aliases: AI326420; AU019811; DNA-PKcs; DNAPDcs; DNAPK; DNPK1; DOXNPH; dxnph; HYRC; HYRC1; IMD26; p350; p460; PRKDC; scid; slip; XRCC7

UniProt ID: (Human) P78527, (Mouse) P97313

Entrez Gene ID: (Human) 5591, (Mouse) 19090, (Rat) 360748

Function(s)

double-stranded DNA binding protein kinase activity protein serine/threonine kinase activity DNA-dependent protein kinase activity protein binding ATP binding transcription factor binding enzyme binding poly(A) RNA binding nucleotide binding DNA binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups phosphotransferase activity, alcohol group as acceptor molecular_function non-receptor serine/threonine protein kinase protein modifying enzyme

Process(es)

somitogenesis negative regulation of protein phosphorylation B cell lineage commitment pro-B cell differentiation T cell lineage commitment negative regulation of immunoglobulin production positive regulation of immune system process double-strand break repair double-strand break repair via nonhomologous end joining cellular protein modification process brain development heart development cell proliferation intrinsic apoptotic signaling pathway in response to DNA damage response to gamma radiation response to activity telomere capping peptidyl-serine phosphorylation protein destabilization positive regulation of type I interferon production cellular response to insulin stimulus T cell differentiation in thymus immunoglobulin V(D)J recombination T cell receptor V(D)J recombination ectopic germ cell programmed cell death regulation of circadian rhythm positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation rhythmic process spleen development thymus development positive regulation of developmental growth regulation of smooth muscle cell proliferation signal transduction involved in mitotic G1 DNA damage checkpoint double-strand break repair via alternative nonhomologous end joining negative regulation of cellular senescence negative regulation of response to gamma radiation telomere maintenance double-strand break repair via homologous recombination immunoglobulin production DNA repair protein phosphorylation cellular response to DNA damage stimulus response to ionizing radiation phosphorylation lymphocyte differentiation V(D)J recombination

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