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Application
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DRD5 Antibody (720284) in WB
Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of Neuro-2a (Lane 1) and tissue extracts of Rat Brain (Lane 2) and Mouse Brain (Lane 3). The blots were probed with Dopamine Receptors- D5 Rabbit Polyclonal antibody (Product # 720284, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A ~ 35 kDa band corresponding to Dopamine Receptors- D5 was observed. Additionally ~50 kDa ba... View More
Product Details
720284
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide corresponding to Human DRD5 (aa 460-477)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.1% BSA, 30% glycerol
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2633237
Product Specific Information
These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
This antibody is predicted to react with Monkey, Dog, Bovine and Cat.
Target Information
The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: D(1B) dopamine receptor; D(5) dopamine receptor; D1b receptor; D1beta dopamine receptor; Dopamine 5 receptor; Dopamine D5 receptor; dopamine receptor 5; dopamine receptor D1B; DRD 1B; DRD 5; Hd5dr; MGC10601
Gene Aliases: D1B; D5R; DBDR; Drd-5; DRD1B; DRD1L2; DRD5; Gpcr1
UniProt ID: (Human) P21918, (Mouse) Q8BLD9, (Rat) P25115
Entrez Gene ID: (Human) 1816, (Mouse) 13492, (Rat) 25195
synaptic transmission, dopaminergic
response to amphetamine
regulation of systemic arterial blood pressure by vasopressin
norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure
cellular calcium ion homeostasis
adenylate cyclase-activating G-protein coupled receptor signaling pathway
activation of adenylate cyclase activity
adenylate cyclase-activating dopamine receptor signaling pathway
chemical synaptic transmission
learning
associative learning
positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway
transmission of nerve impulse
negative regulation of NAD(P)H oxidase activity
wound healing
response to cocaine
positive regulation of adenylate cyclase activity
negative regulation of blood pressure
regulation of female receptivity
sensitization
phospholipase C-activating dopamine receptor signaling pathway
long term synaptic depression
cellular response to catecholamine stimulus
reactive oxygen species metabolic process
negative regulation of protein kinase activity
dopamine receptor signaling pathway
mating behavior
visual learning
negative regulation of cell migration
positive regulation of cAMP biosynthetic process
regulation of long-term neuronal synaptic plasticity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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