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            • Primary Antibodies ›
            • Desmin Antibodies

            Invitrogen

            Desmin Monoclonal Antibody (D33)

            Advanced Verification
            11 Published Figures
            75 References
            View all (69) Desmin antibodies

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            Datasheet
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            Catalog # MA5-13259
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            Datasheet
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            Cite Desmin Monoclonal Antibody (D33)

            • Antibody Testing Data (9)
            • Published Figures (11)
            • Advanced Verification (3)
            Application
            Desmin Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 23

            Desmin Antibody (MA5-13259) in ICC/IF

            Immunofluorescence analysis of desmin was performed using 70% confluent log phase RD cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Desmin Monoclonal Antibody (D33) (Product # MA5-13259) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32... View More
            View Product

            Desmin Antibody in Immunocytochemistry (ICC/IF)
            Desmin Antibody in Immunocytochemistry (ICC/IF)
            Desmin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Desmin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Desmin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Desmin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Desmin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Desmin Antibody in Western Blot (WB)
            Desmin Antibody in Western Blot (WB)
            Desmin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
            Desmin Antibody in Immunocytochemistry (ICC/IF)
            Desmin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
            Desmin Antibody in Immunohistochemistry (Frozen) (IHC (F))
            Desmin Antibody in Immunohistochemistry (IHC)
            Desmin Antibody in Immunohistochemistry (IHC)
            Desmin Antibody in Immunohistochemistry (IHC)
            Desmin Antibody in Immunohistochemistry (IHC)
            Desmin Antibody in Immunohistochemistry (IHC)
            Desmin Antibody in Immunohistochemistry (IHC)
            Desmin Antibody in Immunohistochemistry (IHC)
            Desmin Antibody
            Desmin Antibody
            Desmin Antibody

            Product Details

            MA5-13259

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:1,000
            -

            Immunohistochemistry (IHC)

            -
            View 53 publications 53 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            1:50-1:100
            View 1 publication 1 publication

            Immunohistochemistry (Frozen) (IHC (F))

            -
            View 2 publications 2 publications

            Immunocytochemistry (ICC/IF)

            1:10-1:100
            View 16 publications 16 publications

            Flow Cytometry (Flow)

            -
            View 3 publications 3 publications
            Product Specifications

            Species Reactivity

            Bovine, Dog, Chicken, Cat, Hamster, Human, Non-human primate, Rat

            Published species

            Cat, Chicken, Dog, Human, Mouse, Non-human primate, Rabbit, Rat, Sheep

            Host/Isotype

            Mouse / IgG1, kappa

            Class

            Monoclonal

            Type

            Antibody

            Clone

            D33

            Immunogen

            Desmin from human muscle
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.2 mg/mL

            Purification

            Protein A/G

            Storage buffer

            PBS, pH 7.4, with 0.05% BSA

            Contains

            0.05% sodium azide

            Storage conditions

            4°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_11000611

            Product Specific Information

            MA5-13259 targets Desmin in IF and IHC (P) applications and shows reactivity with Bovine, Canine, Chicken, Feline, Hamster, Human, Non-human primate, and Rat samples.

            The MA5-13259 immunogen is desmin from human muscle.

            Target Information

            Desmin (approximately 53 kDa) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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            Immunohistochemistry
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            Bioinformatics

            Protein Aliases: CMD1I; Desmin; desmin-like protein; FLJ12025; FLJ39719; FLJ41013; FLJ41793; intermediate filament protein; MFM1; mutant desmin p.K241E; SCPNK

            View more View less

            Gene Aliases: CSM1; CSM2; DES; I79_018932; LGMD2R

            View more View less

            UniProt ID: (Bovine) O62654, (Dog) Q5XFN2, (Human) P17661, (Rat) P48675

            View more View less

            Entrez Gene ID: (Bovine) 280765, (Chicken) 395906, (Dog) 497091, (Human) 1674, (Rat) 64362, (Chinese hamster) 100764990

            View more View less

            Function(s)

            cytoskeletal protein binding identical protein binding protein binding structural constituent of cytoskeleton structural molecule activity

            Process(es)

            intermediate filament organization intermediate filament polymerization muscle contraction cytoskeleton organization regulation of heart contraction muscle filament sliding
            It has to be done as per old AB suggested Products section.
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