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            • Primary Antibodies ›
            • H3K4me2 Antibodies
            Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

            Invitrogen

            H3K4me2 Recombinant Superclonal™ Antibody, ChIP-Verified

            Advanced Verification
            1 Published Figure
            3 References
            View all (32) H3K4me2 antibodies
            Datasheet
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            Datasheet
            Protocols
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            Cite H3K4me2 Recombinant Superclonal™ Antibody, ChIP-Verified

            • Antibody Testing Data (8)
            • Published Figures (1)
            • Advanced Verification (3)
            Application
            H3K4me2 Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 12

            H3K4me2 Antibody (710796) in ICC/IF

            Immunofluorescence was performed on fixed and permeabilized Hela cells for detection of Histone H3K4me2 using Anti-Histone H3K4me2 Recombinant Rabbit Superclonal™ Antibody (Product # 710796, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Panel a) shows representative cells that were stained for detection and localization of Histone H3K4me2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mounta... View More
            View Product

            H3K4me2 Antibody in Immunocytochemistry (ICC/IF)
            H3K4me2 Antibody in Western Blot (WB)
            H3K4me2 Antibody in Western Blot (WB)
            H3K4me2 Antibody in Western Blot (WB)
            H3K4me2 Antibody in ChIP Assay (ChIP)
            H3K4me2 Antibody in ChIP Assay (ChIP)
            H3K4me2 Antibody in Peptide array (ARRAY)
            H3K4me2 Antibody in CUT&RUN (C&R)
            H3K4me2 Antibody in Western Blot (WB)
            H3K4me2 Antibody
            H3K4me2 Antibody
            H3K4me2 Antibody

            Product Details

            710796

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            0.5-1 µg/mL
            View 1 publication 1 publication

            Immunohistochemistry (IHC)

            -
            View 1 publication 1 publication

            Immunocytochemistry (ICC/IF)

            1 µg/mL
            -

            ChIP assay (ChIP)

            1-5 µg
            View 1 publication 1 publication

            Peptide Array (Array)

            0.25 µg/mL
            -

            CUT&RUN (C&R)

            1:100
            -
            Product Specifications

            Species Reactivity

            Human, Yeast

            Published species

            C. elegans, Human, Mouse

            Host/Isotype

            Rabbit / IgG

            Expression System

            Expi293

            Class

            Recombinant Superclonal

            Type

            Antibody

            Immunogen

            Methylated peptide (Lys4) corresponding to human Histone H3 (aa 2-10)
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.5 mg/mL

            Purification

            Protein A

            Storage buffer

            PBS, pH 7.2

            Contains

            0.09% sodium azide

            Storage conditions

            Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

            Shipping conditions

            Wet ice

            RRID

            AB_2532765

            Product Specific Information

            Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

            Target Information

            Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: Di histone H3/c; di-methyl H3; di-methyl histone H3; Di-Methyl-H3 K4; Di-Methyl-H3 Lys4; Di-Methyl-Histone H3 K4; Di-Methyl-Histone H3 Lys4; dimethyl H3; Dimethyl Histone H3; H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 K4me2; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3/b; H3/d; H3/f; H3/j; H3K4; H3Lys4me2; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; Histone 3; Histone H3; Histone H3.1; Histone H3.2; Histone H3.3; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o; Histone H3K4; Histone H3K4me2; methyl Histone 3; methyl Histone H3

            View more View less

            Gene Aliases: BUR5; H3; H3-3A; H3-3B; H3.2; H3.3A; H3.3B; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3F1K; H3F2; H3F3; H3F3A; H3F3B; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; HHT1; HHT2; HIST1H3A; HIST1H3B; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D; N2749; PP781; SIN2; YBR010W; YBR0201; YNL031C

            View more View less

            UniProt ID: (Human) P84243, (Baker's yeast) P61830, (Human) P68431, (Human) Q71DI3

            View more View less

            Entrez Gene ID: (Human) 3020, (Baker's yeast) 852295, (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961

            View more View less

            Function(s)

            RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding protein binding nucleosomal DNA binding histone binding protein heterodimerization activity DNA binding cupric reductase activity structural constituent of chromatin cadherin binding involved in cell-cell adhesion histone

            Process(es)

            chromatin silencing at rDNA nucleosome assembly DNA replication-independent nucleosome assembly brain development blood coagulation response to hormone positive regulation of cell growth gene silencing by RNA telomere organization cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic chromatin organization cellular copper ion homeostasis aerobic respiration rRNA transcription sexual sporulation resulting in formation of a cellular spore global genome nucleotide-excision repair positive regulation of defense response to virus by host DNA replication-dependent nucleosome assembly protein heterotetramerization regulation of gene silencing cell-cell adhesion xenophagy
            It has to be done as per old AB suggested Products section.
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            Performance Guarantee

            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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