Save $650 & get a lab-themed hidden object puzzle

Save and claim

Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Productos
    • Anticuerpos
    • Oligonucleótidos, cebadores, sondas y genes
    • Ensayos de PCR en tiempo real TaqMan
    • Medios de cultivos celulares
    • Productos químicos
    • Columnas y cartuchos de cromatografía
    • Equipo de laboratorio
    • Material de plástico y suministros para laboratorio
    • Microplacas
    • Productos más ecológicos
    • Ver todas las categorías de productos
  • Applications
    • Bioprocesamiento
    • Cultivo celular y transfección
    • Terapia celular y génica
    • Cromatografía
    • Pruebas moleculares
    • Soluciones digitales
    • Extracción y análisis de ADN y ARN
    • Espectroscopía, análisis elemental y de isótopos
    • Ver todas las aplicaciones y técnicas
  • Servicios
    • Servicios 360° de CDMO y CRO
    • Servicios de CDMO
    • Servicios de CRO
    • Servicios personalizados
    • Servicios empresariales
    • Servicios de leasing y financiación
    • Servicios de instrumentos
    • Informática de laboratorio
    • OEM y oferta comercial
    • Servicios de formación
    • Unity Lab Services
    • Ver todos los servicios
  • Ayuda y soporte técnico
    • Regístrese para obtener una cuenta
    • Cómo hacer un pedido
    • Asistencia para el instrumental
    • Centros de soporte técnico
    • Centros de formación
    • Vea todos los temas de ayuda y soporte técnico
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Ofertas especiales
  • Contacto
  • Pedido rápido
  • Estado del pedido y seguimiento
  • Documentos y certificados
Thermo Fisher Scientific Logo

Search Thermo Fisher Scientific

Anticuerpos Secundarios
  • Buscar
  • Popular Product Areas
  • Ensayos TaqMan
  • Expresión génica
  • Anticuerpos primarios
  • Anticuerpos Secundarios
  • Controles de isotipo
  • Proteínas y péptidos
  • Kits ELISA
  • SNP Genotyping
  • Todos los documentos y soporte
  • Certificados
  • SDS
  • Preguntas frecuentes sobre productos
  • Manuales y Protocolos
    Search button Close
            Host
            Target Species
            Conjugate
            • Estado del pedido
            • Pedido rápido
            • Sign in
              Sign in
              Don't have an account ? Create Account
              • Cuenta
              • Pedidos
              • Connect: laboratorio, datos, aplicaciones
              • Productos y proyectos personalizados
              • Services Central

            Disclaimer

            Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

            Loading ...
            • Secondary Antibodies ›
            • Donkey anti-Mouse IgG Secondary Antibodies

            Invitrogen

            Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488

            8 Published Figures
            306 References
            View all (72) Donkey anti-Mouse IgG antibodies
            Datasheet
            Create Citation
            Questions & Answers
            Tech Support
            Datasheet
            Create Citation
            Questions & Answers
            Tech Support

            Cite Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488

            • Antibody Testing Data (7)
            • Published Figures (8)
            Application
            Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 15

            Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A32766) in ICC/IF

            Immunofluorescent analysis of HuC/D and MAP-2 in Rat cortical neurons. Gibco Rat Cortex Neurons (Product # A1084001) were thawed and grown according to protocol using B-27 Plus Neurobasal Culture System (Product # A3653401) and GlutaMAX (Product # 35050061) for two weeks before processing with the Image-IT Fixation/Permeablization kit (Product # R37602) according to protocol. Cells were blocked with 3% BSA ... View More
            View Product

            Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
            Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
            Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
            Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
            Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
            Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
            Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
            Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
            Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
            Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
            Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody in Immunohistochemistry (Paraffin) (IHC (P))

            Product Details

            A32766

            Applications
            Tested Dilution

            Western Blot (WB)

            0.1-0.4 µg/mL

            Immunocytochemistry (ICC/IF)

            1-10 µg/mL
            Product Specifications

            Species Reactivity

            Mouse

            Host/Isotype

            Donkey / IgG

            Class

            Polyclonal

            Type

            Secondary Antibody

            Immunogen

            Gamma Immunoglobins Heavy and Light chains

            Conjugate

            Alexa Fluor™ Plus 488
            • Alexa Fluor 350 
            • Alexa Fluor 488 
            • Alexa Fluor 546 
            • Alexa Fluor 555 
            • Alexa Fluor 568 
            • Alexa Fluor 594 
            • Alexa Fluor 647 
            • Alexa Fluor 680 
            • Alexa Fluor 790 
            • Alexa Fluor Plus 405 
            • Alexa Fluor Plus 555 
            • Alexa Fluor Plus 594 
            • Alexa Fluor Plus 647 
            • Alexa Fluor Plus 680 
            • Alexa Fluor Plus 800 
            • AP
            • Biotin 
            • FITC
            • HRP
            • TRITC
            • Request custom conjugation

            Excitation/Emission Max

            493/518 nm View spectra spectra

            Form

            Liquid

            Concentration

            2 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            proprietary buffer, pH 6.5

            Contains

            0.016% Methylisothiazolone, 0.016% Bromonitrodioxane

            Storage conditions

            4°C, store in dark

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2762823

            Target

            IgG

            Cross Adsorption

            Against bovine IgG, goat IgG, chicken IgG, guinea pig IgG, hamster IgG, horse IgG, sheep IgG, rabbit IgG, rat IgG, human IgG

            Antibody Form

            Whole Antibody

            Product Specific Information

            To minimize cross-reactivity, the donkey anti-mouse IgG whole antibodies have been cross-adsorbed against IgG from bovine, goat, chicken, guinea pig, hamster, horse, sheep, rabbit, rat, and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

            Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically

            Specificity: This antibody binds to heavy chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody does not bind non-immunoglobulin mouse serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep.

            Target Information

            Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

            Have you cited this product in a publication?
            Let us know so we can reference it here.
            Cite this product
            It has to be done as per old AB suggested Products section.
            guarantee_icon

            Performance Guarantee

            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

            Learn more
            help_icon

            We're here to help

            Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

            Contact tech support
            Pedidos Plus Icon Minus Icon
            • Estado del pedido
            • Ayuda con el pedido
            • Pedido rápido
            • Centro de suministros
            • eProcurement (B2B)
            Asistencia Plus Icon Minus Icon
            • Ayuda y soporte técnico
            • Contacto
            • Centros de soporte técnico
            • Documentos y certificados
            • Informar de un problema del sitio web
            Recursos Plus Icon Minus Icon
            • Centros de formación
            • Promociones
            • Eventos y seminarios web
            • Redes sociales
            Acerca de Thermo Fisher Plus Icon Minus Icon
            • Acerca de nosotros
            • Carreras profesionales
            • Inversores
            • Noticias
            • Responsabilidad social
            • Marcas registradas
            Nuestra cartera Plus Icon Minus Icon
            • Thermo Scientific
            • Applied Biosystems
            • Invitrogen
            • Gibco
            • Ion Torrent
            • Fisher Scientific
            • Unity Lab Services
            • Patheon
            • PPD
            • Terms & Conditions
            • Privacy Information Center
            • Price & Freight Policy
            • Preferencias sobre cookies

              Su elección con respecto a las cookies en este sitio

              Nosotros y nuestras empresas afiliadas y proveedores utilizamos cookies y tecnologías similares para gestionar nuestros sitios web, reconocer a los visitantes de nuestros sitios web, proporcionar un inicio de sesión seguro, recopilar estadísticas para optimizar las funciones del sitio web y ofrecer contenido adaptado a sus intereses. Haga clic en “Aceptar todas” para aceptar todas las cookies e ir directamente al sitio web; haga clic en “Rechazar todas” para rechazar todas las cookies excepto las estrictamente necesarias para el funcionamiento de este sitio web (cookies obligatorias) o haga clic en “Administrar configuraciones” para ver las descripciones detalladas de los tipos de cookies y elegir si acepta ciertas cookies mientras visita el sitio web.
            © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
            spain flag icon
            Spain

            Your items have has been added!


            Host server : magellan-search-green-56756dfb8b-nml4b:80/100.66.75.22:80.
            git-commit: de6905f249d25e22729e613aef254bb544bd8b7c
            git-url: https://github.com/thermofisher/magellan-search
            git-branch: release/2.31.1-2025.06.25-1.0