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Invitrogen

E-cadherin Monoclonal Antibody (ECCD-1)

Name: E-cadherin Monoclonal Antibody (ECCD-1)
Brand: Invitrogen
SKU: 131800
Price: 590.0 USD
Availability: InStock

1 Published Figure

15 References

View all (173) E-cadherin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (1)

Application

E-cadherin Antibody in Western Blot (WB)

FIGURE: 1 / 1

E-cadherin Antibody (13-1800) in WB

Frontiers in molecular neuroscience 2022 - No change was found in the protein expression level of G-actin. Protein expression level of Cx26, beta-actin (A) , and gamma-actin (E) in P0 control and P0 KD groups. beta-actin and gamma-actin protein expression level in cochlear of Cx26 KD mouse models. The ratio of G-actin/F-actin in P0 control and P0 KD group (B) respectively. Western blotting F-actin and G-actin in Western blot results of protein expre... View More

PMID: 35345836

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Product Details

13-1800

Applications

Tested Dilution

Publications

Western Blot (WB)

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Neutralization (Neu)

View 3 publications 3 publications

Inhibition Assays (IA)

200 µg/mL

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Human, Mouse, Rat, Tag

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

ECCD-1

Immunogen

Mouse teratocarcinoma cell line F9.

View immunogen

Conjugate

Unconjugated

Form

Lyophilized

Concentration

2.0 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533004

Product Specific Information

Source: Monoclonal antibody was obtained by fusing the P3-X63-Ag8 mouse myeloma cell line with spleen cells of Wistar rat after immunization with mouse teratocarcinoma cell line F9.

Specificity: This antibody specifically reacts with mouse E-cadherin. This antibody inhibits E-cadherin dependent cell-cell contact.

Cross reactivity: This antibody does not react with human, bovine, chicken or dog E-cadherin.

Storage: 4°C This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.

Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

Target Information

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

F-Actin Dysplasia Involved in Organ of Corti Deformity in Gjb2 Knockdown Mouse Model.

Frontiers in molecular neuroscience

Liu XZ,Jin Y,Chen S,Xu K,Xie L,Qiu Y,Wang XH,Sun Y,Kong WJ

13-1800 was used in Immunohistochemistry and Western Blotting to indicate that Cx26 is involved in the maturation of the cytoskeleton during the development of the OC at the early postnatal stage.

Wed Mar 30 00:00:00 UTC 2022

F-Actin Dysplasia Involved in Organ of Corti Deformity in Gjb2 Knockdown Mouse Model.

Frontiers in molecular neuroscience

Liu XZ,Jin Y,Chen S,Xu K,Xie L,Qiu Y,Wang XH,Sun Y,Kong WJ

13-1800 was used in Immunohistochemistry and Western Blotting to indicate that Cx26 is involved in the maturation of the cytoskeleton during the development of the OC at the early postnatal stage.

Wed Mar 30 00:00:00 UTC 2022

Identification of MYC-Dependent Transcriptional Programs in Oncogene-Addicted Liver Tumors.

Cancer research

Kress TR,Pellanda P,Pellegrinet L,Bianchi V,Nicoli P,Doni M,Recordati C,Bianchi S,Rotta L,Capra T,Ravà M,Verrecchia A,Radaelli E,Littlewood TD,Evan GI,Amati B

131800 was used in immunohistochemistry - paraffin section to find MYC-dependent regulatory events present in a fully developed carcinoma

Wed Jun 15 00:00:00 UTC 2016

Lgr5 Marks Neural Crest Derived Multipotent Oral Stromal Stem Cells.

Stem cells (Dayton, Ohio)

Boddupally K,Wang G,Chen Y,Kobielak A

13-1800 was used in immunohistochemistry - frozen section to elucidate neural crest derived multipotent oral stromal stem cells marked by Lgr5

Tue Mar 01 00:00:00 UTC 2016

E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells.

Stem cells and development

Moore RN,Cherry JF,Mathur V,Cohen R,Grumet M,Moghe PV

13-1800 was used in blocking or activating experiment and immunocytochemistry to describe a protocol to differentiate hESCs into neural stem cells, which can then be differentiated into neuronal restricted cells.

Sun Jan 01 00:00:00 UTC 2012

E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells.

Stem cells and development

Moore RN,Cherry JF,Mathur V,Cohen R,Grumet M,Moghe PV

Sun Jan 01 00:00:00 UTC 2012

E-Cadherin regulates neural stem cell self-renewal.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Karpowicz P,Willaime-Morawek S,Balenci L,DeVeale B,Inoue T,van der Kooy D

13-1800 was used in blocking or activating experiment to study E-cadherin in neural stem cells

Wed Mar 25 00:00:00 UTC 2009

Connexin expression and gap junctional coupling in human cumulus cells: contribution to embryo quality.

Journal of cellular and molecular medicine

Wang HX,Tong D,El-Gehani F,Tekpetey FR,Kidder GM

Fri May 01 00:00:00 UTC 2009

Connexin expression and gap junctional coupling in human cumulus cells: contribution to embryo quality.

Journal of cellular and molecular medicine

Wang HX,Tong D,El-Gehani F,Tekpetey FR,Kidder GM

Fri May 01 00:00:00 UTC 2009

Self-organization and branching morphogenesis of primary salivary epithelial cells.

Tissue engineering

Wei C,Larsen M,Hoffman MP,Yamada KM

13-1800 was used in blocking or activating experiment to investigate the regeneration of submandibular gland epithelium

Sun Apr 01 00:00:00 UTC 2007

Conditional targeting of E-cadherin in skin: insights into hyperproliferative and degenerative responses.

Proceedings of the National Academy of Sciences of the United States of America

Tinkle CL,Lechler T,Pasolli HA,Fuchs E

Tue Jan 13 00:00:00 UTC 2004

Conditional targeting of E-cadherin in skin: insights into hyperproliferative and degenerative responses.

Proceedings of the National Academy of Sciences of the United States of America

Tinkle CL,Lechler T,Pasolli HA,Fuchs E

Tue Jan 13 00:00:00 UTC 2004

Cadherin-mediated cell adhesion and tissue segregation: qualitative and quantitative determinants.

Developmental biology

Duguay D,Foty RA,Steinberg MS

13-1800 was used in blocking assay and flow cytometry to examine cadherin-subtype-specific binding specificities.

Wed Jan 15 00:00:00 UTC 2003

The fate of E- and P-cadherin during the early stages of apoptosis.

Cell death and differentiation

Schmeiser K,Grand RJ

13-1800 was used in western blot to investigate cisplatin-induced apoptosis in human embryo retinoblasts.

Thu Apr 01 00:00:00 UTC 1999

The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.

Development (Cambridge, England)

Takeichi M

Fri Apr 01 00:00:00 UTC 1988

The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.

Development (Cambridge, England)

Takeichi M

Fri Apr 01 00:00:00 UTC 1988

The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.

Development (Cambridge, England)

Takeichi M

Fri Apr 01 00:00:00 UTC 1988

Bioinformatics

Protein Aliases: ARC-1; cadherin e; Cadherin-1; cadherin-E; CD324; E-cadherin; Ecadherin; Epithelial cadherin; Uvomorulin

Gene Aliases: AA960649; Cdh1; E-cad; Ecad; L-CAM; Um; UVO

UniProt ID: (Mouse) P09803

Entrez Gene ID: (Mouse) 12550

Function(s)

glycoprotein binding calcium ion binding protein binding beta-catenin binding protein phosphatase binding protein domain specific binding ankyrin binding GTPase activating protein binding gamma-catenin binding metal ion binding cell adhesion molecule binding cadherin

Process(es)

in utero embryonic development trophectodermal cell differentiation epithelial cell morphogenesis cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules synapse assembly sensory perception of sound single organismal cell-cell adhesion calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules protein metabolic process negative regulation of cell-cell adhesion regulation of protein localization regulation of water loss via skin adherens junction organization positive regulation of transcription factor import into nucleus regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of transcription, DNA-templated regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation protein homooligomerization intestinal epithelial cell development salivary gland cavitation regulation of branching involved in salivary gland morphogenesis bicellular tight junction assembly cellular response to amino acid stimulus cellular response to lithium ion cellular response to indole-3-methanol protein localization to plasma membrane establishment of protein localization to plasma membrane negative regulation of canonical Wnt signaling pathway cochlea development regulation of protein localization to cell surface regulation of neuron migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

E-cadherin Monoclonal Antibody (ECCD-1)

E-cadherin Antibody (13-1800) in WB

Product Details

13-1800

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Neutralization (Neu)

Inhibition Assays (IA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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