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Application
FIGURE: 1 / 3
EBF2 Antibody (PA5-115155) in ICC/IF
Immunocytochemistry analysis of EBF2 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115155). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-115155
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human EBF2(Accession Q9HAK2), corresponding to amino acid residues V532-M575.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2899791
Product Specific Information
Antibody detects endogenous levels of total EBF2.
Target Information
Early B-cell factor 2 (EBF2) is a gene located on chromosome 8p11.23, encoding a transcription factor involved in the regulation of gene expression during neural and adipose tissue development. EBF2 belongs to the COE family of transcription factors and plays a pivotal role in cellular differentiation processes. In the nervous system, EBF2 is essential for the development and function of neurons, particularly in regions like the olfactory system. Additionally, EBF2 is involved in adipogenesis, where it regulates genes responsible for the differentiation of brown adipocytes, contributing to thermogenesis and energy metabolism. Dysfunction or altered expression of EBF2 can impact neurological function and metabolic homeostasis, potentially influencing conditions such as obesity, diabetes, and neurodevelopmental disorders. Research into EBF2 is exploring its role in these physiological contexts and the implications of its regulatory mechanisms, offering insights into potential therapeutic approaches.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Collier, Olf and EBF 2; Early B-cell factor 2; EBF-2; MET-mesencephalon-olfactory TF1; MET-mesencephalon-olfactory transcription factor 1; metencephalon-mesencephalnon-olfactory transcription factor 1; Metencephalon-mesencephalon-olfactory transcription factor 1; O/E-3; OLF-1/EBF-LIKE 3; Transcription factor COE2
Gene Aliases: COE2; D14Ggc1e; EBF-2; EBF2; Mmot1; O/E-3; OE-3
UniProt ID: (Human) Q9HAK2, (Mouse) O08792
Entrez Gene ID: (Human) 64641, (Rat) 361060, (Mouse) 13592
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