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ECT2 Antibody (PA5-18166) in ICC/IF
Immunocytochemistry analysis of ECT2 using ECT2 Polyclonal Antibody (Product # PA5-18166) in paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing strong nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-18166
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (AENSVLTSTTGRT) corresponding to the N-terminus amino acids of ECT2
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_10984642
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.
Target Information
The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epithelial cell transforming sequence 2 oncogene; Epithelial cell-transforming sequence 2 oncogene; FLJ10461; MGC138291; Protein ECT2
Gene Aliases: ARHGEF31; ECT2
UniProt ID: (Human) Q9H8V3
Entrez Gene ID: (Human) 1894
signal transducer activity
guanyl-nucleotide exchange factor activity
Rho guanyl-nucleotide exchange factor activity
GTPase activator activity
protein binding
Rho GTPase binding
protein homodimerization activity
G-protein modulator
protein-binding activity modulator
guanyl-nucleotide exchange factor
cell morphogenesis
cytokinesis
protein transport
activation of protein kinase activity
positive regulation of cytokinesis
regulation of Rho protein signal transduction
intracellular signal transduction
positive regulation of protein import into nucleus
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of GTPase activity
positive regulation of neuron differentiation
regulation of protein kinase activity
regulation of small GTPase mediated signal transduction
protein homooligomerization
regulation of attachment of spindle microtubules to kinetochore
cellular response to hydrogen peroxide
bicellular tight junction assembly
cellular response to calcium ion
cellular response to ionizing radiation
activation of GTPase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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